Anti-HDAC8 Antibody (CAB5829)[KO Validated]

SKU:
CAB5829
  • KO Validated Antibodies 1 Anti-HDAC8 Antibody CAB5829KO Validated
  • KO Validated Antibodies 1 Anti-HDAC8 Antibody CAB5829KO Validated
  • KO Validated Antibodies 1 Anti-HDAC8 Antibody CAB5829KO Validated
  • KO Validated Antibodies 1 Anti-HDAC8 Antibody CAB5829KO Validated
  • KO Validated Antibodies 1 Anti-HDAC8 Antibody CAB5829KO Validated
€129 - €349

Description

Technical Manual

Anti-HDAC8 Antibody [KO Validated]

Product Name:Anti-HDAC8 Antibody [KO Validated]
Product SKU:CAB5829
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
Sequence:

MEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV

Gene ID:55869
Swiss Prot:Q9BY41
Synonyms:HDAC8; CDA07; CDLS5; HD8; HDACL1; MRXS6; RPD3; WTS
Calculated MW:15kDa/16kDa/17kDa/28kDa/29kDa/31kDa/41kDa
Observed MW:42kDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence
Positive Samples:293T, Mouse lung
Cellular Location:Cytoplasm, Nucleus

Product Images

Anti-HDAC8 Antibody [KO Validated]  (CAB5829)

Western blot analysis of extracts of various cell lines, using HDAC8 Antibody (CAB5829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Anti-HDAC8 Antibody [KO Validated]  (CAB5829)

Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) HeLa cells, using HDAC8 antibody (CAB5829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Anti-HDAC8 Antibody [KO Validated]  (CAB5829)

Immunohistochemistry of paraffin-embedded rat testis using HDAC8 antibody (CAB5829) at dilution of 1:100 (40x lens).

Anti-HDAC8 Antibody [KO Validated]  (CAB5829)

Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC8 antibody (CAB5829) at dilution of 1:100 (40x lens).

Anti-HDAC8 Antibody [KO Validated]  (CAB5829)

Immunohistochemistry of paraffin-embedded human breast cancer using HDAC8 antibody (CAB5829) at dilution of 1:100 (40x lens).

Uniprot Information

UniProt Protein Function:HDAC8: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. Belongs to the histone deacetylase family. HD type 1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; nuclear chromosome; histone deacetylase complex; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function:NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding

Biological Process: chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; sister chromatid cohesion; mitotic cell cycle; chromatin modification; negative regulation of transcription from RNA polymerase II promoter

Disease: Wilson-turner X-linked Mental Retardation Syndrome

NCBI Summary:Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:Q9BY41
NCBI GenInfo Identifier:29839394
NCBI Gene ID:55869
NCBI Accession:Q9BY41.2
UniProt Secondary Accession:Q9BY41,Q86VC8, Q9NP76, Q9NYH4, A6ND12, A6ND61, A6NET3 A6NJR3, A8MQ62, B4DKN0, B4DV22,
UniProt Related Accession:Q9BY41
Molecular Weight:377
NCBI Full Name:Histone deacetylase 8
NCBI Synonym Full Names:histone deacetylase 8
NCBI Official Symbol:HDAC8
NCBI Official Synonym Symbols:HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
NCBI Protein Information:histone deacetylase 8; histone deacetylase-like 1
UniProt Protein Name:Histone deacetylase 8
UniProt Gene Name:HDAC8
UniProt Entry Name:HDAC8_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
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