Anti-Bcl10 Antibody (CAB1106)

Antibody Name:	Anti-Bcl10 Antibody [KO Validated]
Antibody SKU:	CAB1106
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human Bcl10 (NP_003912.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	MCF-7, DU145, A-549, BT-474, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human Bcl10 (NP_003912.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEPT APSL TEED LTEV KKDA LENL RVYL CEKI IAER HFDH LRAK KILS REDT EEIS CRTS SRKR AGKL LDYL QENP KGLD TLVE SIRR EKTQ NFLI QKIT DEVL KLRN IKLE HLKG LKCS SCEP FPDG ATNN LSRS NSDE SNFS EKLR ASTV MYHP EGES STTP FFST NSSL NLPV LEVG RTEN TIFS STTL PRPG DPGA PPLP PDLQ LEEE GTCA NSSE MFLP LRSR TVSR Q
Gene ID:	8915
Uniprot:	O95999
Cellular Location:	Cytoplasm, Membrane raft, perinuclear region
Calculated MW:	26kDa
Observed MW:	33kDa

Synonyms:

BCL10, CARMEN, CIPER, CLAP, IMD37, c-E10, mE10

Background:

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	Bcl-10: a ubiquitous pro-apoptotic protein that promotes pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Self-associates via CARD-CARD interactions; forms a tight complex with MALT1. Interacts with other CARD-proteins such as CARD9, CARD10, CARD11 and CARD14. Binds caspase-9 with its C-terminal domain. Interacts with TRAF2 and BIRC2/c-IAP2. Defects in BCL10 are involved in various types of cancer.
UniProt Protein Details:	Protein type:Apoptosis; Tumor suppressor; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 1p22 Cellular Component: CBM complex; protein complex; lysosome; immunological synapse; cytosol; lipid raft; T cell receptor complex; cytoplasmic microtubule; perinuclear region of cytoplasm; cytoplasm; plasma membrane; lipopolysaccharide receptor complex; nucleus Molecular Function:protein C-terminus binding; protein kinase B binding; ubiquitin binding; protease binding; protein self-association; transcription coactivator activity; transcription factor binding; protein kinase binding; NF-kappaB binding; protein binding; enzyme binding; ubiquitin protein ligase binding; kinase activator activity; kinase binding Biological Process: cell death; response to fungus; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; B cell apoptosis; T cell receptor signaling pathway; interleukin-6 biosynthetic process; activation of NF-kappaB transcription factor; negative regulation of mature B cell apoptosis; response to molecule of bacterial origin; protein homooligomerization; response to food; adaptive immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; protein oligomerization; positive regulation of protein ubiquitination; lymphotoxin A biosynthetic process; regulation of T cell receptor signaling pathway; neural tube closure; toll-like receptor signaling pathway; innate immune response; cellular defense response; positive regulation of mast cell cytokine production; positive regulation of T cell activation; immunoglobulin mediated immune response; positive regulation of phosphorylation Disease: Follicular Lymphoma, Susceptibility To, 1; Mesothelioma, Malignant; Gastric Lymphoma, Primary; Testicular Germ Cell Tumor; Immunodeficiency 37
NCBI Summary:	This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq, Jul 2008]
UniProt Code:	O95999
NCBI GenInfo Identifier:	4502379
NCBI Gene ID:	8915
NCBI Accession:	NP_003912.1
UniProt Secondary Accession:	O95999,Q5VUF1,
UniProt Related Accession:	O95999
Molecular Weight:	26,252 Da
NCBI Full Name:	B-cell lymphoma/leukemia 10
NCBI Synonym Full Names:	B-cell CLL/lymphoma 10
NCBI Official Symbol:	BCL10
NCBI Official Synonym Symbols:	CLAP; mE10; CIPER; c-E10; CARMEN
NCBI Protein Information:	B-cell lymphoma/leukemia 10; hCLAP; cCARMEN; cellular-E10; cellular homolog of vCARMEN; CARD-containing proapoptotic protein; CARD containing molecule enhancing NF-kB; CARD-containing apoptotic signaling protein; caspase-recruiting domain-containing prote
UniProt Protein Name:	B-cell lymphoma/leukemia 10
UniProt Synonym Protein Names:	B-cell CLL/lymphoma 10; Bcl-10; CARD-containing molecule enhancing NF-kappa-B; CARD-like apoptotic protein; hCLAP; CED-3/ICH-1 prodomain homologous E10-like regulator; CIPER; Cellular homolog of vCARMEN; cCARMEN; Cellular-E10; c-E10; Mammalian CARD-containing adapter molecule E10
Protein Family:	B-cell lymphoma/leukemia
UniProt Gene Name:	BCL10
UniProt Entry Name:	BCL10_HUMAN

	Western blot analysis of extracts of various cell lines, using Bcl10 antibody (CAB1106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and Bcl10 knockout (KO) HeLa cells, using Bcl10 antibody (CAB1106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies	ELISA Kits
Anti-Bcl10 Antibody (CAB4520)	Human Bcl-10 (B-cell Leukemia/Lymphoma 10) ELISA Kit (HUES01741)
BCL10 Antibody (PACO06001)	Human B-cell lymphoma/leukemia 10 (BCL10) ELISA Kit
BCL10 Antibody (PACO07990)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)