Anti-CAPG Antibody (CAB7324)[KO Validated]

SKU:
CAB7324
  • KO Validated Antibodies 1 Anti-CAPG Antibody CAB7324KO Validated
  • KO Validated Antibodies 1 Anti-CAPG Antibody CAB7324KO Validated
  • KO Validated Antibodies 1 Anti-CAPG Antibody CAB7324KO Validated
  • KO Validated Antibodies 1 Anti-CAPG Antibody CAB7324KO Validated
  • KO Validated Antibodies 1 Anti-CAPG Antibody CAB7324KO Validated
€129 - €349

Description

Technical Manual

Anti-CAPG Antibody [KO Validated]

Product Name:Anti-CAPG Antibody [KO Validated]
Product SKU:CAB7324
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2).
Sequence:

MYTAIPQSGSPFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGGVESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGGKSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFISRMQYAPNTQVEILPQGHESPIFKQFFKDWK

Gene ID:822
Swiss Prot:P40121
Synonyms:CAPG; AFCP; HEL-S-66; MCP
Calculated MW:36kDa/38kDa
Observed MW:38kDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry
Positive Samples:THP-1, HL-60, Mouse lung, Mouse spleen, Rat heart, Rat lung
Cellular Location:Cytoplasm, Melanosome, Nucleus

Product Images

Anti-CAPG Antibody [KO Validated] (CAB7324)

Western blot analysis of extracts of various cell lines, using CAPG Antibody (CAB7324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Anti-CAPG Antibody [KO Validated] (CAB7324)

Western blot analysis of extracts of various cell lines, using CAPG Antibody (CAB7324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Anti-CAPG Antibody [KO Validated] (CAB7324)

Western blot analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using CAPG antibody (CAB7324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Anti-CAPG Antibody [KO Validated] (CAB7324)

Immunohistochemistry of paraffin-embedded human lung cancer using CAPG Antibody (CAB7324) at dilution of 1:100 (40x lens).

Anti-CAPG Antibody [KO Validated] (CAB7324)

Immunohistochemistry of paraffin-embedded human liver cancer using CAPG Antibody (CAB7324) at dilution of 1:100 (40x lens).

Uniprot Information

UniProt Protein Function:CAPG: Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. Belongs to the villin/gelsolin family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: F-actin capping protein complex; melanosome; nucleus

Molecular Function:actin binding

Biological Process: protein complex assembly; cell projection biogenesis; barbed-end actin filament capping

NCBI Summary:This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:P40121
NCBI GenInfo Identifier:313104088
NCBI Gene ID:822
NCBI Accession:P40121.2
UniProt Secondary Accession:P40121,Q53SA7, B8ZZS7, D6W5K8,
UniProt Related Accession:P40121
Molecular Weight:348
NCBI Full Name:Macrophage-capping protein
NCBI Synonym Full Names:capping protein (actin filament), gelsolin-like
NCBI Official Symbol:CAPG
NCBI Official Synonym Symbols:MCP; AFCP; HEL-S-66
NCBI Protein Information:macrophage-capping protein; macrophage capping protein; gelsolin-like capping protein; actin regulatory protein CAP-G; actin-regulatory protein CAP-G; epididymis secretory protein Li 66
UniProt Protein Name:Macrophage-capping protein
UniProt Synonym Protein Names:Actin regulatory protein CAP-G
Protein Family:Protein
UniProt Gene Name:CAPG
UniProt Entry Name:CAPG_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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