Anti-Vimentin Antibody (CAB11952)[KO Validated]

SKU:
CAB11952
  • KO Validated Antibodies 1 Anti-Vimentin Antibody CAB11952KO Validated
  • KO Validated Antibodies 1 Anti-Vimentin Antibody CAB11952KO Validated
  • KO Validated Antibodies 1 Anti-Vimentin Antibody CAB11952KO Validated
  • KO Validated Antibodies 1 Anti-Vimentin Antibody CAB11952KO Validated
  • KO Validated Antibodies 1 Anti-Vimentin Antibody CAB11952KO Validated
€129 - €349

Description

Technical Manual

Anti-Vimentin Antibody [KO Validated]

Product Name:Anti-Vimentin Antibody [KO Validated]
Product SKU:CAB11952
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human Vimentin (NP_003371.2).
Sequence:

MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRT

Gene ID:7431
Swiss Prot:P08670
Synonyms:CTRCT30; HEL113; Vimentin; VIM; vimentin
Calculated MW:53kDa
Observed MW:57kDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:Jurkat, HeLa
Cellular Location:Cytoplasm

Product Images

Anti-Vimentin Antibody [KO Validated] (CAB11952)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (CAB11952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Anti-Vimentin Antibody [KO Validated] (CAB11952)

Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (CAB11952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Anti-Vimentin Antibody [KO Validated] (CAB11952)

Immunohistochemistry of paraffin-embedded human tonsil using Vimentin antibody (CAB11952) at dilution of 1:150 (40x lens).

Anti-Vimentin Antibody [KO Validated] (CAB11952)

Immunohistochemistry of paraffin-embedded human stomach using Vimentin antibody (CAB11952) at dilution of 1:150 (40x lens).

Anti-Vimentin Antibody [KO Validated] (CAB11952)

Immunofluorescence analysis of L929 cells using [KO] Vimentin antibody (CAB11952) at dilution of 1:100. Blue: DAPI for nuclear staining.

Uniprot Information

UniProt Protein Function:Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function:protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30

NCBI Summary:This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
UniProt Code:P08670
NCBI GenInfo Identifier:55977767
NCBI Gene ID:7431
NCBI Accession:P08670.4
UniProt Related Accession:P08670
Molecular Weight:
NCBI Full Name:Vimentin
NCBI Synonym Full Names:vimentin
NCBI Official Symbol:VIM 
NCBI Protein Information:vimentin
UniProt Protein Name:Vimentin
Protein Family:Vimentin
UniProt Gene Name:VIM 
UniProt Entry Name:VIME_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
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