Anti-TFAP4 Antibody (CAB16727)

Antibody Name:	Anti-TFAP4 Antibody [KO Validated]
Antibody SKU:	CAB16727
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	A synthetic Peptide of human TFAP4

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HT-29, SGC-7901, 22Rv1, HeLa

Immunogen:	A synthetic Peptide of human TFAP4
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	7023
Uniprot:	Q01664
Cellular Location:	Nucleus
Calculated MW:	38kDa
Observed MW:	42kDa

Synonyms:	TFAP4, AP-4, bHLHc41
Background:

UniProt Protein Function:	AP-4: Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'- CAGCTG-3'.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 16p13 Cellular Component: nucleus; transcriptional repressor complex Molecular Function:DNA binding; histone deacetylase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription coactivator activity Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of DNA binding; negative regulation of transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; protein complex assembly
NCBI Summary:	Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM, Jul 2009]
UniProt Code:	Q01664
NCBI GenInfo Identifier:	1729833
NCBI Gene ID:	7023
NCBI Accession:	Q01664.2
UniProt Secondary Accession:	Q01664,O60409,
UniProt Related Accession:	Q01664
Molecular Weight:	38,726 Da
NCBI Full Name:	Transcription factor AP-4
NCBI Synonym Full Names:	transcription factor AP-4
NCBI Official Symbol:	TFAP4
NCBI Official Synonym Symbols:	AP-4; bHLHc41
NCBI Protein Information:	transcription factor AP-4
UniProt Protein Name:	Transcription factor AP-4
UniProt Synonym Protein Names:	Activating enhancer-binding protein 4; Class C basic helix-loop-helix protein 41; bHLHc41
Protein Family:	Transcription factor
UniProt Gene Name:	TFAP4
UniProt Entry Name:	TFAP4_HUMAN

	Western blot analysis of extracts of various cell lines, using TFAP4 antibody (CAB16727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Western blot analysis of extracts from normal (control) and TFAP4 knockout (KO) HeLa cells, using TFAP4 antibody (CAB16727) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60S.
	Immunofluorescence analysis of C6 cells using TFAP4 antibody (CAB16727) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using TFAP4 antibody (CAB16727) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

TFAP4 Antibody (PACO12710)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)