In vivo Functional Grade Antibodies

Figure 1. Quality control for different lots of PD-1 [RMP1-14] antibody. All the in vivo antibodies undergo extensive quality control ensuring extreme lot-to-lot reproducibility. Endotoxin data for eight lots demonstrate that all lots were below the specification of ≤ 0.5 EU/mg as determined by the limulus amebocyte lysate (LAL) method (Fig. 1A). Analysis by size exclusion chromatography visualized by superimposing chromatograms for all nine lots, with overlapping retention times and minimal baseline noise (Fig. 1B). All lots show high purity as shown by non-reducing and reducing SDS-PAGE (Fig. 1C and 1D).