Anti-Cytokeratin 8 Antibody (CAB1024)[KO Validated]

SKU:
CAB1024
  • KO Validated Antibodies 1 Anti-Cytokeratin 8 Antibody CAB1024KO Validated
  • KO Validated Antibodies 1 Anti-Cytokeratin 8 Antibody CAB1024KO Validated
  • KO Validated Antibodies 1 Anti-Cytokeratin 8 Antibody CAB1024KO Validated
  • KO Validated Antibodies 1 Anti-Cytokeratin 8 Antibody CAB1024KO Validated
€129 - €349

Description

Technical Manual

Anti-Cytokeratin 8 Antibody [KO Validated]

Product Name:Anti-Cytokeratin 8 Antibody [KO Validated]
Product SKU:CAB1024
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human Cytokeratin 8 (NP_002264.1).
Sequence:

MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK

Gene ID:3856
Swiss Prot:P05787
Synonyms:KRT8; CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; keratin 8
Calculated MW:53kDa/56kDa
Observed MW:53kDa
Reactivity:Human, Mouse
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence
Positive Samples:293T, NIH/3T3
Cellular Location:Cytoplasm, Nucleus, Nucleus matrix, nucleoplasm

Product Images

Anti-Cytokeratin 8 Antibody [KO Validated] (CAB1024)

Western blot analysis of extracts of various cell lines, using Cytokeratin 8 antibody (CAB1024) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-Cytokeratin 8 Antibody [KO Validated] (CAB1024)

Western blot analysis of extracts from normal (control) and Cytokeratin 8 knockout (KO) HeLa cells, using Cytokeratin 8 antibody (CAB1024) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Anti-Cytokeratin 8 Antibody [KO Validated] (CAB1024)

Immunohistochemistry of paraffin-embedded human appendix using [KO Validated] Cytokeratin 8 Rabbit pAb (CAB1024) at dilution of 1:100 (40x lens).

Anti-Cytokeratin 8 Antibody [KO Validated] (CAB1024)

Immunofluorescence analysis of HeLa cells using Cytokeratin 8 antibody (CAB1024) at dilution of 1:100. Blue: DAPI for nuclear staining.

Uniprot Information

UniProt Protein Function:K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: dystrophin-associated glycoprotein complex; nucleoplasm; intermediate filament cytoskeleton; nuclear matrix; costamere; cytoplasm; keratin filament; intermediate filament; intercellular junction; Z disc; nucleus; sarcolemma

Molecular Function:protein binding; protein complex binding; structural molecule activity

Biological Process: tumor necrosis factor-mediated signaling pathway; viral reproduction; response to other organism; sarcomere organization; response to hydrostatic pressure

Disease: Cirrhosis, Familial

NCBI Summary:This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:P05787
NCBI GenInfo Identifier:90110027
NCBI Gene ID:3856
NCBI Accession:P05787.7
UniProt Secondary Accession:P05787,Q14099, Q14716, Q14717, Q53GJ0, Q6DHW5, Q6GMY0 Q6P4C7, Q96J60, A8K4H3, B0AZN5, F8VXB4,
UniProt Related Accession:P05787
Molecular Weight:483
NCBI Full Name:Keratin, type II cytoskeletal 8
NCBI Synonym Full Names:keratin 8
NCBI Official Symbol:KRT8
NCBI Official Synonym Symbols:K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
NCBI Protein Information:keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
UniProt Protein Name:Keratin, type II cytoskeletal 8
UniProt Synonym Protein Names:Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Protein Family:Keratin
UniProt Gene Name:KRT8
UniProt Entry Name:K2C8_HUMAN
View AllClose

Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
View AllClose