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Cholesterol Assay Kit - InformationAssay Genie's Cholesterol Assay (Colorimetric/Fluorometric) uses a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to total cholesterol concentration in the sample.
ApplicationsFor quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.
Cholesterol Assay Kit - Key Features
- Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays.
- Convenient. Room temperature assay. No 37°C heater is needed.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Cholesterol Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 20 mL Enzyme Mix: 120 uL Dye Reagent: 120 mL Standard: 1 mL 300 mg/dL cholesterol|
|Kit Requires||Pipetting (multi-channel) devices, 96-well plate and plate reader|
|Method of Detection||OD570nm, or FL530/585nm|
|Detection Limit||OD, FL: 1, 0.2 mg/dL|
|Samples||Serum, plasma etc|
|Protocol Length||40 min|
|Storage||Store reagents at -20°C|
|Shelf Life||12 months|