Phospholipid Assay Kit (Colorimetric/Fluorometric) (BA0143)
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Phospholipid Assay Kit
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.Assay Genie's phospholipid assay kit provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.
ApplicationsFor quantitative determination of phospholipid and evaluation of drug effects on its metabolism.
- Sensitive. Use 20 uL samples. Linear detection range: colorimetric assay 3 - 200 uM, fluorimetric assay 0.6 - 20 uM phospholipid.
Phospholipid Assay Kit Information
|Kit Includes:||Assay Buffer: 10 mL, PLD Enzyme: 120 mL, Enzyme Mix: Dried Dye Reagent: 120 mL Standard: 400 mL 2 mM phosphatidylcholine|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader|
|Method of Detection:||OD570nm, or FL530/585nm|
|Detection Limit:||OD, FL: 3, 0.6 uM|
|Protocol Length:||20 min|
|Storage:||Store all components at -20°C|
|Shelf Life:||6 months|