Phospholipid Assay Kit (Colorimetric/Fluorometric) (BA0143)

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SKU:
BA0143
€683

Description

ELISA Kit Technical ManualMSDS

Phospholipid Assay Kit

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.

Assay Genie's phospholipid assay kit provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.

Applications

For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.

Key Features

  • Sensitive. Use 20 uL samples. Linear detection range: colorimetric assay 3 - 200 uM, fluorimetric assay 0.6 - 20 uM phospholipid.

Phospholipid Assay Kit Information

Kit Includes: Assay Buffer: 10 mL, PLD Enzyme: 120 mL, Enzyme Mix: Dried Dye Reagent: 120 mL Standard: 400 mL 2 mM phosphatidylcholine
Kit Requires: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader
Method of Detection: OD570nm, or FL530/585nm
Detection Limit: OD, FL: 3, 0.6 uM
Samples: Biological
Species: All
Protocol Length: 20 min
Size: 100 tests
Storage: Store all components at -20°C
Shelf Life: 6 months
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Additional Information

Product type:
Assay
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