- Metabolism Assays
- Galactose Assay Kit (Colorimetric/Fluorometric) (BA0112)
Galactose Assay Kit
Galactose (C6H12O6) is a monosaccharide that is found in dairy products, sugar beets, gums and mucilages. It is also synthesized in mammals, where it forms part of glycolipids and glycoproteins in several tissues. It forms the disaccharide lactose when combined with glucose. Simple, direct and high-throughput assays for galactose determination find wide applications.
Assay Genie's galactose assay uses specific enzyme-coupled reactions to form a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the galactose concentration in the sample.
For quantitative determination of galactose and its metabolism.
- Use as little as 20 uL samples. Linear detection range in 96-well plate: 10 to 1000 uM galactose for colorimetric assays and 10 to 100 uM for fluorimetric assays.
Galactose Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL Enzyme Mix: Dried Dye Reagent: 120 uL Standard: 1 mL|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, optical density plate reader; black 96-well plates and fluorescence plate reader|
|Method of Detection:||OD570nm, or FL530/585nm|
|Samples:||Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc|
|Protocol Length:||20 min|
|Storage:||Store all components at -20°C|
|Shelf Life:||12 months|