Description
Galactose Assay Kit (Colorimetric/Fluorometric) (BA0112) (BA0112)
The Galactose Assay Kit (Colorimetric/Fluorometric) (SKU: BA0112) provides a simple, direct and high-throughput method for measuring galactose. Galactose is a monosaccharide found in dairy products, sugar beet and gums, and forms lactose when combined with glucose. The assay uses specific enzyme-coupled reactions to form a coloured product; the colour intensity at 570 nm or fluorescence at 530/585 nm is directly proportional to the galactose concentration. The procedure can be run in either colorimetric or fluorometric format and suits food, beverage and biological applications.
| Product Name: | Galactose Assay Kit (Colorimetric/Fluorometric) (BA0112) |
| SKU: | BA0112 |
| Detection Method: | Colorimetric (570 nm) / Fluorometric (530/585 nm) |
| Detection Range: | Colorimetric 10 - 1000 uM; fluorometric 10 - 100 uM galactose |
| Sample Type: | Serum, plasma, urine, saliva, milk, culture medium, food, beverages and other biological samples |
| Species Reactivity: | All |
| Assay Time: | 20 minutes |
| Kit Size: | 100 Assays |
| Equipment Required: | Microplate reader |
| Storage: | -20C |
| Shelf Life: | 12 months after receipt |
| Shipping: | Gel Pack |
A colorimetric or fluorometric assay for galactose across a wide range of matrices, using as little as 20 uL of sample. The Enzyme Mix is supplied dried and reconstituted before use.
- Uses as little as 20 uL of sample
- Colorimetric linear range 10 - 1000 uM and fluorometric range 10 - 100 uM galactose
- Suitable for food, beverage and biological samples
- Direct assay of galactose in serum, plasma, urine, saliva, milk, culture medium and other biological samples
- Studying the effects of drugs on galactose metabolism
- Galactose determination in food and beverage products
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Equilibrate components to room temperature and reconstitute the Enzyme Mix with 120 uL distilled water (stable for 3 months at -20C); keep on ice during use. |
| 2 | Serum and plasma can be assayed directly. Clear milk samples by mixing 600 uL milk with 100 uL 6 N HCl, centrifuging, then neutralising 300 uL supernatant with 50 uL 6 N NaOH (dilution factor 1.36). |
| 3 | Standards: prepare 400 uL of 1000 uM standard by mixing 40 uL of 10 mM standard with 360 uL distilled water, then dilute per the table. Transfer 20 uL of each standard and 20 uL of each sample into separate wells of a clear flat-bottom 96-well plate. |
| 4 | Reaction: for each well mix 85 uL Assay Buffer, 1 uL Enzyme Mix and 1 uL Dye Reagent. Transfer 80 uL Working Reagent into each well, tap to mix and incubate 20 minutes at room temperature. |
| 5 | Read optical density at 570 nm (550-585 nm). For the fluorometric assay, use 10 - 100 uM standards and a black plate and read fluorescence at 530/585 nm. |
Subtract the water blank from the standard values and plot delta-OD or delta-RFU against standard concentration. [Galactose] = (ODSAMPLE - ODH2O) / Slope x n (uM) for colorimetry, or (RFU_SAMPLE - RFU_H2O) / Slope x n (uM) for fluorimetry, where n is the dilution factor. 1 mM galactose equals 18 mg/dL, 0.018% or 180 ppm.
| Component | Quantity | Storage |
| Assay Buffer | 10 mL | -20C |
| Enzyme Mix | Dried | -20C |
| Dye Reagent | 120 uL | -20C |
| Standard | 1 mL | -20C |
