Oxaloacetate Assay Kit (Colorimetric/Fluorometric) (BA0139)
Oxaloacetate Assay Kit
Oxaloacetate (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO2 with pyruvate or phosphoenolpyruvate.Assay Genie's Oxaloacetate Assay Kit provides a simple, direct and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the oxaloacetate concentration in the sample.
ApplicationsFor quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism.
- Sensitive and accurate. Linear detection range: 7 to 400 uM oxaloacetate for colorimetric assays and 1 to 40 uM for fluorimetric assays.
Oxaloacetate Assay Kit Information:
|Kit Includes:||Developer: 10 mL ODC Enzyme: 120 mL Dye Reagent: 120 mL Oxaloacetate Standard: Dried|
|Kit Requires:||Pipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes.|
|Method of Detection:||OD570nm, or FL530/585nm|
|Detection Limit:||OD, FL: 4, 1 uM|
|Samples:||Plasma, serum, tissue and culture media|
|Protocol Length:||20 min|
|Storage:||Store all kit components at -20 °C|
|Shelf Life:||6 months|