Creatine Assay Kit (Colorimetric/Fluorometric) (BA0095)
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Creatine Assay Kit - InformationAssay Genie' creatine assay is based on enzymatic reactions leading to formation of a pink colored product. The optical density at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the creatine concentration in the sample.
ApplicationsFor quantitative determination of creatine and evaluation of drug effects on creatine metabolism.
Creatine Assay Kit - Key Features
- High sensitivity and wide linear range. Use 10 sample. Linear detection range 4 to 1000 (colorimetric) or 0.5 to 50 (fluorimetric).
- Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes.
Creatine Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 20 mL Enzyme A: 120 mL Enzyme B: 220 mL Standard: 400 mL 20 mM creatine Dye Reagent: 220 mL|
|Kit Requires||Pipeting devices, and clear flat-bottom 96-well plates and optical density plate reader for colorimetric assays; black flatbottom 96-well plate and fluorescence intensity plate reader for fluorimetric assays.|
|Method of Detection||OD570nm, or FL530/585nm|
|Samples||Serum, plasma, urine, saliva etc|
|Protocol Length||30 min|
|Storage||Store all components at -20°C|
|Shelf Life||12 months|