Creatine Assay Kit (Colorimetric/Fluorometric) (BA0095)
Creatine Assay Kit - InformationAssay Genie' creatine assay is based on enzymatic reactions leading to formation of a pink colored product. The optical density at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the creatine concentration in the sample.
ApplicationsFor quantitative determination of creatine and evaluation of drug effects on creatine metabolism.
Creatine Assay Kit - Key Features
- High sensitivity and wide linear range. Use 10 sample. Linear detection range 4 to 1000 (colorimetric) or 0.5 to 50 (fluorimetric).
- Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes.
Creatine Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 20 mL Enzyme A: 120 mL Enzyme B: 220 mL Standard: 400 mL 20 mM creatine Dye Reagent: 220 mL|
|Kit Requires||Pipeting devices, and clear flat-bottom 96-well plates and optical density plate reader for colorimetric assays; black flatbottom 96-well plate and fluorescence intensity plate reader for fluorimetric assays.|
|Method of Detection||OD570nm, or FL530/585nm|
|Samples||Serum, plasma, urine, saliva etc|
|Protocol Length||30 min|
|Storage||Store all components at -20°C|
|Shelf Life||12 months|
More DetailsCREATINE is present in vertebrates and helps to supply energy to muscle. In humans and animals, approximately half of creatine originates from food (mainly from fresh meat). Creatine supplementation has been investigated as a possible therapeutic approach for the treatment of muscular, neuromuscular, neurological and neurodegenerative diseases. Simple, direct and automation-ready procedures for measuring creatine are popular in research and drug discovery