Citrate Assay Kit (Colorimetric or Fluorometric) (BA0090)
Citrate Assay Kit - InformationAssay Genie's Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the citrate concentration in the sample.
ApplicationsFor quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.
Citrate Assay Kit - Key Features
- Fast and sensitive. Linear detection range: 4 to 400 citrate for colorimetric assays and 0.5 to 40 for fluorimetric assays.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Citrate Assay Kit - Data Sheet
|Kit Includes||Developer: 10 mL CL Enzyme: Dried Dye Reagent: 120 mL ODC Enzyme: 120 mL Citrate Standard: 500 mL|
|Kit Requires||Pipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes.|
|Method of Detection||OD570nm, or FL530/585nm|
|Detection Limit||OD, FL: 4, 0.5 .|
|Samples||Serum, plasma, agriculture etc|
|Protocol Length||20 min|
|Storage||Store all kit components at -20 °C|
|Shelf Life||6 months|
More DetailsCITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis.