- Metabolism Assays
- Maltose Assay Kit (Colorimetric or Fluorometric) (BA0132)
Maltose Assay Kit - Information
MALTOSE (C12H22O11) is a disaccharide, composed of two glucose units linked by an alpha bond. It is produced from the hydrolysis of glycogen or starch, serving as a source of energy for plants and animals. Maltose can be found in foods such as grains, and other processed products.Assay Genie's maltose assay provides a simple, one step assay for measuring maltose. In this assay, maltose is converted to two glucoses, which are then oxidized to form a colored product. The color intensity of the product at 570 nm or fluorescence at Ex/Em = 530/585 nm is directly proportional to maltose concentration in the sample.
ApplicationsQuantitative determination of maltose in various biological samples such as serum, urine, food and beverages, etc.
Maltose Assay Kit - Key Features
- Fast and sensitive. Use of 10 uL sample. Linear detection range 2 to 500 uM maltose in for colorimetric assays and 1 to 50 uM for flourimetric assays.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Maltose Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL, Dye Reagent: 120 uL, Enzyme A: 120 uL, Standard: 1 mL, 5 mM Maltose Enzyme Mix: 120 uL|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate reader|
|Method of Detection:||OD570nm, or FL530/585nm|
|Detection Limit:||2 uM|
|Samples:||Serum, urine, food and beverages, etc.|
|Protocol Length:||60 min|
|Storage:||Store all components at -20°C upon receiving|
|Shelf Life:||6 months|