- Metabolism Assays
- Acetate Assay Kit (Colorimetric and Fluorometric) (BA0140)
Acetate Assay Kit
Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibres.Assay Genie's acetate assay uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.
ApplicationsFor quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism.
- Use as little as 10 uL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays.
Acetate Assay Kit Information:
|Kit Includes:||Assay Buffer: 25 mL, Enzyme A: Dried Developer: 1 mL Enzyme B: Dried Dye Reagent: 120 mL, Standard: 1 mL, ATP: 120 mL|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, optical density plate reader; black 96-well plates and fluorescence plate reader.|
|Method of Detection:||OD570nm, or FL530/585nm|
|Detection Limit:||0.13 mM|
|Samples:||Serum, plasma, food, agriculture and environment|
|Protocol Length:||30 min|
|Storage:||Store all components at -20°C|
|Shelf Life:||6 months|