- Metabolism Assays
- Xanthine Assay Kit (Colorimetric or Fluorometric) (BA0154)
Xanthine Oxidase Assay Kit
Xanthine is a purine base that can be found in most animal tissues and fluids. It is a product in the purine degradation pathway, produced by guanine deaminase from guanine, and by xanthine oxidoreductase from hypoxanthine. Xanthine is degraded to uric acid by xanthine oxidase. Clinically, xanthine and its derivatives act on sleep-inducing adenosine receptors as antagonists. Simple, direct and high-throughput assays for measuring xanthine find wide applications in research and drug discovery.Assay Genie's xanthine assay kit uses a single Working Reagent that combines the xanthine oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to xanthine concentration in the sample.
ApplicationsFor quantitative determination of xanthine and drug effects on its metabolism.
- Sensitive and accurate. Use as little as 10 uL samples. Linear detection range in 96-well plate for 30 minute incubation: 0.01 to 2.5 mM xanthine for colorimetric assays and 3 to 250 uM for fluorimetric assays.
- Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.
- Fast and high-throughput. Assays using 96-well plates and liquid handling system could allow simultaneous processing tens of thousands of samples per day.
Xanthine Oxidase Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL, HRP Enzyme: 120 uL, Standard: 1 mL, 2 mM Xanthine Dye Reagent: 120 uL, XO Enzyme: 100 uL|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, black 96-well plates and plate reader capable of either measuring absorbance between 550-585 nm or fluorescence intensity at ex/em = 530/585 nm.|
|Method of Detection:||OD570nm, FL530/585nm|
|Detection Limit:||FL 3 uM; OD 10 uM|
|Samples:||Cell lysate, serum, and other biological samples.|
|Protocol Length:||30 min|
|Storage:||Store all reagents at -20°C|
|Shelf Life:||6 months|