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Phospholipase D Activity Assay Kit (BA0144)

SKU:
BA0144
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Biological
Research Area:
Lipid Metabolism
Plant & Environmental Stress
Enzyme Activity
Clinical Chemistry
€809
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Description

ELISA Kit Technical ManualMSDS

Phospholipase D Activity Assay

Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers.

Assay Genie's phospholipase D activity assay provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.

Applications

For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

Phospholipase D Activity Assay - Key Features

  • Sensitive. Use 10 uL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.
  • Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.
Kit Includes: Assay Buffer: 10 mL, Dye Reagent: 120 mL, Enzyme Mix: Dried Substrate: 1.5 mL, Calibrator: 400 mL
Kit Requires: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader
Method of Detection: OD570nm, or FL530/585nm
Detection Limit: 0.04 U/L
Samples: Biological
Species: All
Protocol Length: 30 min
Size: 100 tests
Storage: Store all components at -20°C
Shelf Life: 6 months

 

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