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Urease Activity Assay Kit (BA0061)

SKU:
BA0061
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Biological, Environment
Research Area:
Diabetes & Obesity
Plant & Environmental Stress
Enzyme Activity
€779
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Urease Activity Assay - Information

Assay Genie's Urease Activity Assay Kit provides a very sensitive and convenient means to measure urease activity in a variety of samples including soil. In the assay, urease reacts with urea, resulting in the formation of ammonia, which is determined by the Berthelot method at 670nm. The assay is simple, sensitive, stable and high-throughput adaptable

Applications

For quantitative determination of urease activity and evaluation/screen for urease inhibitors.

Urease Activity Assay - Key Features

  • Safe. Non-radioactive assay.
  • Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified.
  • Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
  • Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Urease Activity Assay - Data Sheet

Kit IncludesAssay Buffer: 20 mL (pH 7.0) Reagent A: 12 mL Urea: 1.5 mL Reagent B: 6 mL NH4Cl: 100 mL 50 mM
Kit RequiresPipetting devices, centrifuge tubes, clear flat-bottom 96-well plate.
Method of DetectionOD670nm
Detection Limit0.003 U/L
SamplesBiological, environment etc
SpeciesAll
Protocol Length40 min
Size100 tests
StorageStore all reagent at 4°C
Shelf Life6 months

More Details

UREASE (Amidohydrolase, EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia.(NH2)2)CO + H2)O CO2) + 2NH3). Many gastrointestinal or urinary tract pathogens produce urease. Thus its activity is a useful diagnostic parameter for the presence of pathogens such as Helicobacter pylori. Urease is found in bacteria, yeast, and higher plants. Urease activity is commonly determined in anaerobes of the bovine rumen, human feces and environmental samples such as soils and phytoplanktons.

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