Adipolysis Assay Kit (BA0076)
Adipolysis Assay Kit - InformationAssay Genie's adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample.
ApplicationsFor quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
Adipolysis Assay Kit - Key Features
- Sensitive and accurate. Use as little as 10 samples. Linear detection range in 96-well plate: 0.92 to 100 ug/mL (10 to 1000 ) glycerol for colorimetric assays and 0.2 to 5 ug/mL for fluorimetric assays.
- Rapid and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
- Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates.
Adipolysis Assay Kit -Data Sheet
|Kit Includes||Assay Buffer: 24 mL Enzyme Mix: 500 mL ATP: 250 mL Dye Reagent: 220 mL Standard: 100 mL 100 mM Glycerol|
|Kit Requires||Pipeting devices, centrifuge tubes, appropriate 96- or 384-well plates and plate reader|
|Method of Detection||OD570nm, or FL530/585nm|
|Detection Limit||0.92 ug/mL|
|Samples||Cell culture media|
|Protocol Length||20 min|
|Storage||Store Assay Buffer at 4°C and other reagents at -20°C|
|Shelf Life||12 months|
More DetailsObesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery.