Lactose Assay Kit (BA0126)



ELISA Kit Technical ManualMSDS

Lactose Assay Kit

Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of beta-D-galactose and alpha/beta-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2-8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications.

Assay Genie's lactose assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.


For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.

Key Features

  • Use as little as 20 uL samples. Linear detection range in 96-well plate: 17 to 2000 uM lactose for colorimetric assays and 6 to 100 uM for fluorimetric assays.

Lactose Assay Kit Information

Kit Includes: Assay Buffer: 10 mL, Enzyme Mix: Dried Dye Reagent: 120 uL, Lactase: Dried Standard (20 mM Lactose): 1 mL
Kit Requires: Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, optical density plate reader; black 96-well plates and fluorescence plate reader.
Method of Detection: OD570nm, or FL530/585nm
Detection Limit: 6 uM
Samples: Biological (milk), food and agriculture etc
Species: All
Protocol Length: 30 min
Size: 100 tests
Storage: Store all components at -20°C
Shelf Life: 12 months
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