Isocitrate Assay Kit (Colorimetric) (BA0089)

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SKU:
BA0089
€617

Description

ELISA Kit Technical ManualMSDS

Isocitrate Assay Kit - Information

Assay Genie's isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.

Applications

For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

Isocitrate Assay Kit - Key Features

  • Fast and sensitive. Linear detection range (20 sample): 20 to 5000 for 10 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Isocitrate Assay Kit - Data Sheet

Method of DetectionOD565nm
Detection Limit20
SamplesFood, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
SpeciesAll
Protocol Length10 min
Size100 tests
Shelf Life6 months

More Details

ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.
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