Isocitrate Assay Kit (Colorimetric) (BA0089)
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Isocitrate Assay Kit - InformationAssay Genie's isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.
ApplicationsFor quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
Isocitrate Assay Kit - Key Features
- Fast and sensitive. Linear detection range (20 sample): 20 to 5000 for 10 min reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Isocitrate Assay Kit - Data Sheet
|Method of Detection||OD565nm|
|Samples||Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)|
|Protocol Length||10 min|
|Shelf Life||6 months|