The QuickStep Human D2D D-Dimer ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of D-dimer levels in human samples including serum and plasma. D-dimer is a fibrin degradation product that serves as a biomarker for various medical conditions, particularly in the diagnosis and monitoring of thrombotic disorders such as deep vein thrombosis, pulmonary embolism, and disseminated intravascular coagulation. With its reliable and reproducible results, this ELISA kit is ideal for researchers and healthcare professionals looking to study thrombotic diseases, assess coagulation status, and monitor treatment effectiveness. Understand and quantify D-dimer levels with precision using the QuickStep Human D2D D-Dimer ELISA Kit.
Product Name:
Human D2D (D-Dimer) Quickstep ELISA Kit
SKU:
QSES024
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
53.1 pg/mL
Detection range:
125-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.