The QuickStep Human Fe Ferritin ELISA Kit is specifically designed to accurately detect and measure levels of ferritin in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Ferritin is a key protein involved in iron storage and regulation in the body, making it a vital biomarker for conditions such as iron deficiency anemia, hemochromatosis, and other iron-related disorders. Monitoring ferritin levels can help researchers better understand these conditions and develop targeted therapies.With the QuickStep Human Fe Ferritin ELISA Kit, researchers can confidently study the role of ferritin in various diseases and conditions, enabling advancements in diagnostics and treatment strategies.
Product Name:
Human FE (Ferritin) Quickstep ELISA Kit
SKU:
QSES017
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
24.31 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
