The Mouse Fe-Ferritin ELISA Kit is specifically designed for the precise quantification of Fe-ferritin levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Fe-ferritin is a vital protein responsible for iron storage and regulation in the body. Abnormal levels of Fe-ferritin have been linked to various health conditions, including iron deficiency anemia, hemochromatosis, and neurodegenerative diseases. As such, measuring Fe-ferritin levels can provide valuable insights into these conditions and aid in the development of potential treatments. The Mouse Fe-Ferritin ELISA Kit is a valuable tool for researchers studying iron metabolism, red blood cell production, and iron-related disorders in mice. Its user-friendly protocol and high performance make it an essential asset for laboratories conducting preclinical studies and drug development research.
Product Name:
Mouse FE (Ferritin) ELISA Kit
SKU:
MOES01014
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
