Yes. Every protocol, guide and troubleshooting resource in this hub is freely available with no login or purchase required. Each links to a full step-by-step method with reaction setups, cycling programs and tips.

For cloning, mutagenesis and any application where sequence accuracy matters, use a high-fidelity, proofreading polymerase such as Genie Fusion Ultra High-Fidelity DNA Polymerase. For routine screening or colony PCR where occasional errors are acceptable, a standard Taq polymerase is faster and more economical.

DNA assembly (GenieClone) joins one or more fragments seamlessly and sequence-independently using short homologous overlaps, so there are no leftover restriction scars. TA cloning ligates a PCR product with 3'-A overhangs directly into a complementary T-vector — quick and simple, but not seamless. Choose assembly for multi-fragment or scarless constructs and TA cloning for fast, single-insert work.

The GenieClone site-directed mutagenesis kits can introduce three to five separate mutations in a single reaction in under three hours, and can complete up to two mutations with a single amplification reaction. See the site-directed mutagenesis protocol for primer design and reaction setup.

Yes. Assay Genie's GenieClone and Genie Fusion molecular biology tools — high-fidelity polymerase, T4 DNA ligase, DNA assembly and site-directed mutagenesis kits — are all available in the Molecular Biology range, each with its own protocol linked above.

Yes. Our PhD-level scientific team is happy to help diagnose failed amplifications, empty plates or off-target mutations. Use the site-directed mutagenesis troubleshooting guide for common fixes, or get in touch with your experimental details for tailored support.