Mouse F10 Recombinant Protein (RPES5250)

SKU:
RPES5250
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Mouse
€196

Description

ELISA Kit Technical Manual

Recombinant Mouse Coagulation Factor X/F10 Protein (His Tag)

Background:Mouse coagulation factor X / F10 a member of the peptidase S1 family. The mature F10 is composed mostly of two EGF-like domains, one Gla gamma-carboxy-glutamate domain and one peptidase S1 domain. Factor Xa is a vitamin K-dependent plasma protease that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. The two chains of F10 are formed from a single-chain precursor by the excision of two Arg residues. A single-chain precursor is initially synthesized in the liver. The light and heavy chains are linked together by disulfide bonds. The light chain contains a Gla and two EGF-like domains. The heavy chain corresponds to the serine protease domain. It can form a heterodimer with SERPINA5.
Name:Recombinant Mouse Coagulation Factor X/F10 Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES5250
Synonyms:F10;Coagulation factor X;Stuart factor
Species:Mouse
Expression host:Human Cells
Sequence:Gly21-Asn481
Accession:O88947
Mol Mass:55.1 kDa
AP Mol Mass:55 kDa
Tag:C-6His
Purity:> 95 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM MES,?150mM NaCl?and?1mM CaCl2 pH7.5..
Reconstitution:Please refer to the printed manual for detailed information.
NCBI Summary:This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
UniProt Code:O88947
NCBI GenInfo Identifier:4322473
NCBI Gene ID:14058
NCBI Accession:
UniProt Related Accession:O88947
Molecular Weight:55 kDa
NCBI Full Name:factor X, partial
NCBI Synonym Full Names:coagulation factor X
NCBI Official Symbol:F10  
NCBI Official Synonym Symbols:fX; Cf10  
NCBI Protein Information:coagulation factor X
UniProt Protein Name:
Protein Family:Factor X-activator
UniProt Gene Name:
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