Mouse coagulation factor X / F10 a member of the peptidase S1 family. The mature F10 is composed mostly of two EGF-like domains, one Gla gamma-carboxy-glutamate domain and one peptidase S1 domain. Factor Xa is a vitamin K-dependent plasma protease that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. The two chains of F10 are formed from a single-chain precursor by the excision of two Arg residues. A single-chain precursor is initially synthesized in the liver. The light and heavy chains are linked together by disulfide bonds. The light chain contains a Gla and two EGF-like domains. The heavy chain corresponds to the serine protease domain. It can form a heterodimer with SERPINA5.
Product Name:
Mouse F10 Recombinant Protein (RPES5250)
Product Code:
RPES5250
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
F10,Coagulation factor X,Stuart factor
Accession:
O88947
Sequence:
Gly21-Asn481
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Coagulation factor X is produced by our Mammalian expression system and the target gene encoding Gly21-Asn481 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
55.1 kDa
AP Mol Mass:
55kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM MES,?150mM NaCl?and?1mM CaCl2 pH7.5..
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
