Mouse CD34 Recombinant Protein (RPES0948)
- SKU:
- RPES0948
- Product Type:
- Recombinant Protein
- Species:
- Mouse
Frequently bought together:
Description
system_update_altDatasheet
Mouse CD34 Recombinant Protein
Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.
| Product Name: | Mouse CD34 Recombinant Protein (RPES0948) |
| Product Code: | RPES0948 |
| Size: | 20µg |
| Species: | Mouse |
| Expressed Host: | HEK293 Cells |
| Synonyms: | AU040960 |
| Accession: | NP_001104529.1 |
| Sequence: | Met 1-Thr 287 |
| Fusion tag: | C-His |
| Activity: | Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 10E4 cells/well are added to mouse CD34 coated plates (0.8 µg/ml, 100 ?l/well), approximately >30 % will adhere after one hour at 37 ?. |
| Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the extracellular domain of mouse CD34 (NP_001104529.1) (Met 1-Thr 287) was expressed, with a polyhistidine tag at the C-terminus. |
| Purity: | > 98 % as determined by SDS-PAGE |
| Mol Mass: | 28.6 kDa |
| AP Mol Mass: | 50-60 kDa |
| Formulation: | Lyophilized from sterile PBS, pH 7.4 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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