The Human Coupling Factor 6 (CF6) ELISA Kit is specifically developed for the precise measurement of CF6 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for various research purposes.CF6 is an important protein associated with cellular energy production and metabolism. It plays a key role in the proper functioning of mitochondria, the powerhouse of the cell.
Dysregulation of CF6 levels has been linked to metabolic disorders, mitochondrial dysfunction, and various other health conditions, making it a valuable biomarker for studying these diseases and potentially discovering new therapeutic interventions.Overall, the Human CF6 ELISA Kit provides researchers with a reliable tool to investigate the role of CF6 in health and disease, offering valuable insights for biomedical research and potential clinical applications.
Product Name:
Human Coupling Factor 6 (CF6) ELISA Kit
Product Code:
HUDL00539
Size:
96 Assays
Target:
CF6
Synonyms:
ATP5J, ATP5A, ATP5, ATPM, ATP Synthase,H+ Transporting,Mitochondrial Fo Complex,Subunit F6, ATP synthase-coupling factor 6, mitochondrial
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
