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Human ACO2/Aconitase 2 Recombinant Protein (RPES2394)

SKU:
RPES2394
Product type:
Recombinant Protein
Host species:
Baculovirus-Insect Cells
Reactivity:
Human
€535

Description

ELISA Kit Technical Manual

Recombinant Human ACO2/Aconitase 2 Protein (His & GST Tag)

Background:Interleukin 10 receptor, beta subunit (IL10RB/IL0RB) also known as Cytokine receptor family 2 member 4, Interleukin0 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin0 receptor. IL10RB/IL0RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. Defects in IL10RB/IL0RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Name:Recombinant Human ACO2/Aconitase 2 Protein (His & GST Tag)
Size:10µg
Assay Genie SKU:RPES2394
Synonyms:ACONM;ICRD
Species:Human
Expression host:Baculovirus-Insect Cells
Sequence:Gln 28-Gln 780
Accession:Q99798
Mol Mass:110 kDa
AP Mol Mass:110 kDa
Tag:N-His & GST
Purity:> 85 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg of the protein as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 0.3mM DTT
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:ACO2: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Monomer. Belongs to the aconitase/IPM isomerase family.
UniProt Protein Details:

Protein type:Lyase; EC 4.2.1.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: mitochondrion; mitochondrial matrix; nucleus

Molecular Function:iron ion binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; 3 iron, 4 sulfur cluster binding

Biological Process: isocitrate metabolic process; cellular metabolic process; generation of precursor metabolites and energy; tricarboxylic acid cycle; citrate metabolic process

Disease: Infantile Cerebellar-retinal Degeneration; Optic Atrophy 8

NCBI Summary:The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008]
UniProt Code:Q99798
NCBI GenInfo Identifier:6686275
NCBI Gene ID:50
NCBI Accession:Q99798.2
UniProt Related Accession:Q99798
Molecular Weight:
NCBI Full Name:Aconitate hydratase, mitochondrial
NCBI Synonym Full Names:aconitase 2
NCBI Official Symbol:ACO2  
NCBI Official Synonym Symbols:ICRD; OCA8; OPA9; ACONM; HEL-S-284  
NCBI Protein Information:aconitate hydratase, mitochondrial
UniProt Protein Name:Aconitate hydratase, mitochondrial
UniProt Synonym Protein Names:Citrate hydro-lyase
Protein Family:Aconitate hydratase
UniProt Gene Name:ACO2  
UniProt Entry Name:ACON_HUMAN