Anti-GMPS Antibody (CAB6606)

Antibody Name:	Anti-GMPS Antibody
Antibody SKU:	CAB6606
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 394-693 of human GMPS (NP_003866.1).

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF-7, SKOV3, HL-60, U-251MG, Mouse heart, Mouse kidney, Mouse brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 394-693 of human GMPS (NP_003866.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DTEL IRKL REEG KVIE PLKD FHKD EVRI LGRE LGLP EELV SRHP FPGP GLAI RVIC AEEP YICK DFPE TNNI LKIV ADFS ASVK KPHT LLQR VKAC TTEE DQEK LMQI TSLH SLNA FLLP IKTV GVQG DCRS YSYV CGIS SKDE PDWE SLIF LARL IPRM CHNV NRVV YIFG PPVK EPPT DVTP TFLT TGVL STLR QADF EAHN ILRE SGYA GKIS QMPV ILTP LHFD RDPL QKQP SCQR SVVI RTFI TSDF MTGI PATP GNEI PVEV VLKM VTEI KKIP GISR IMYD LTSK PPGT TEWE
Gene ID:	8833
Uniprot:	P49915
Cellular Location:	Cytoplasm
Calculated MW:	65kDa/76kDa
Observed MW:	80kDa

Synonyms:

GMPS, GATD7

Background:

In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP.

UniProt Protein Function:	GMPS: Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division. A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.
UniProt Protein Details:	Protein type:Oncoprotein; Nucleotide Metabolism - purine; Xenobiotic Metabolism - drug metabolism - other enzymes; Ligase; EC 6.3.5.2 Chromosomal Location of Human Ortholog: 3q24 Cellular Component: cytoplasm; cytosol Molecular Function:GMP synthase (glutamine-hydrolyzing) activity; GMP synthase activity Biological Process: purine base biosynthetic process; purine ribonucleoside monophosphate biosynthetic process Disease: Leukemia, Acute Myeloid
NCBI Summary:	In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]
UniProt Code:	P49915
NCBI GenInfo Identifier:	1708072
NCBI Gene ID:	8833
NCBI Accession:	P49915.1
UniProt Secondary Accession:	P49915,A8K639, B4DXV7, F8W720,
UniProt Related Accession:	P49915
Molecular Weight:	65,929 Da
NCBI Full Name:	GMP synthase
NCBI Synonym Full Names:	guanine monophosphate synthase
NCBI Official Symbol:	GMPS
NCBI Protein Information:	GMP synthase [glutamine-hydrolyzing]
UniProt Protein Name:	GMP synthase [glutamine-hydrolyzing]
UniProt Synonym Protein Names:	GMP synthetase; Glutamine amidotransferase
UniProt Gene Name:	GMPS
UniProt Entry Name:	GUAA_HUMAN

	Western blot analysis of extracts of various cell lines, using GMPS antibody (CAB6606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GMPS antibody (CAB6606). Western blot was performed from the immunoprecipitate using GMPS antibody (CAB6606) at a dilition of 1:1000.

Antibodies

GMPS Antibody (PACO09505)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)