Recombinant Human ATG10 Protein (His&T7 Tag) (RPES3103)

SKU:
RPES3103
€361

Description

ELISA Kit Technical Manual

Recombinant Human ATG10 Protein (His&T7 Tag)

Background:Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.
Name:Recombinant Human ATG10 Protein (His&T7 Tag)
Size:10µg
Assay Genie SKU:RPES3103
Synonyms:Ubiquitin-Like-Conjugating Enzyme ATG10; Autophagy-Related Protein 10; APG10-Like; ATG10
Species:Human
Expression host:E.coli
Sequence:Met 1-Thr190
Accession:Q9H0Y0
Mol Mass:24.5 kDa
AP Mol Mass:30 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 8.0.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.2.-; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytosol; intracellular

Molecular Function:APG12 conjugating enzyme activity

Biological Process: autophagy; macroautophagy; positive regulation of protein modification process; protein amino acid lipidation; protein modification by small protein conjugation

NCBI Summary:Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9H0Y0
NCBI GenInfo Identifier:62286633
NCBI Gene ID:83734
NCBI Accession:Q9H0Y0.1
UniProt Secondary Accession:Q9H0Y0,Q6PIX1, Q9H842, B2RE09,
UniProt Related Accession:Q9H0Y0
Molecular Weight:14,217 Da
NCBI Full Name:Ubiquitin-like-conjugating enzyme ATG10
NCBI Synonym Full Names:autophagy related 10
NCBI Official Symbol:ATG10
NCBI Official Synonym Symbols:APG10; APG10L; pp12616
NCBI Protein Information:ubiquitin-like-conjugating enzyme ATG10
UniProt Protein Name:Ubiquitin-like-conjugating enzyme ATG10
UniProt Synonym Protein Names:Autophagy-related protein 10; APG10-like
Protein Family:Ubiquitin-like-conjugating enzyme
UniProt Gene Name:ATG10
UniProt Entry Name:ATG10_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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