Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.
Product Name:
Human ATG10 Recombinant Protein (RPES3103)
Product Code:
RPES3103
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Ubiquitin-Like-Conjugating Enzyme ATG10, Autophagy-Related Protein 10, APG10-Like, ATG10
Accession:
Q9H0Y0
Sequence:
Met 1-Thr190
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Autophagy Related 10 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Thr190 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
24.5 kDa
AP Mol Mass:
30 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
