Human IF Recombinant Protein (RPPB3753)
- SKU:
- RPPB3753
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P27352
Description
| Product Name: | Human IF Recombinant Protein |
| Product Code: | RPPB3753 |
| Size: | 10µg |
| Species: | Human |
| Target: | IF |
| Synonyms: | Gastric intrinsic factor, Intrinsic factor, INF, IF, GIF, IFMH, TCN3, Cobalamin/Vitamin B-12 binding transport protein. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered pink solution. |
| Formulation: | The protein solution contains 20mM HEPES pH-8.0, 100mM NaCl�and 20% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Intrinsic Factor is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12 in the terminal ileum. Vitamin B12 is essential for erythrocyte maturation and mutations in the Intrinsic Factor may lead to congenital pernicious anemia. Upon entry into the stomach, vitamin B12 binds to one of two B12 binding proteins present in the gastric fluid. In the less acidic environment of the small intestine, these proteins dissociate from the vitamin, allowing it to bind to intrinsic factor and enter the portal circulation through a receptor in the ileal mucosa specific for the B12-intrinsic factor complex.
Intrinsic Factor Human Recombinant produced in baculovirus is a glycosylated, polypeptide chain containing having a molecular mass of 55,000 Dalton. The Intrinsic Factor is fused to a hexa-histidine at the C-terminus and purified by proprietary chromatographic techniques for removal of bound Vitamin B-12.
| UniProt Protein Function: | GIF: Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis. Defects in GIF are the cause of hereditary intrinsic factor deficiency (IFD); also known as congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q13 Cellular Component: extracellular space; lysosomal lumen; microvillus; apical plasma membrane; extracellular region; endosome Molecular Function:cobalamin binding Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process Disease: Intrinsic Factor Deficiency |
| NCBI Summary: | This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P27352 |
| NCBI GenInfo Identifier: | 62906845 |
| NCBI Gene ID: | 2694 |
| NCBI Accession: | P27352.2 |
| UniProt Related Accession: | P27352 |
| Molecular Weight: | |
| NCBI Full Name: | Cobalamin binding intrinsic factor |
| NCBI Synonym Full Names: | cobalamin binding intrinsic factor |
| NCBI Official Symbol: | CBLIF�� |
| NCBI Official Synonym Symbols: | IF; GIF; INF; IFMH; TCN3�� |
| NCBI Protein Information: | cobalamin binding intrinsic factor |
| UniProt Protein Name: | Gastric intrinsic factor |
| UniProt Synonym Protein Names: | Intrinsic factor; IF; INF |
| Protein Family: | Gastric intrinsic factor |
| UniProt Gene Name: | GIF�� |
| UniProt Entry Name: | IF_HUMAN |
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