The Human APOA4 (Apolipoprotein A4) ELISA Kit is specifically designed for the precise detection of apolipoprotein A4 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.Apolipoprotein A4 is a key protein involved in lipid metabolism and plays a crucial role in regulating cholesterol levels and lipid transport in the body. Abnormal levels of APOA4 have been associated with various metabolic disorders, including cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments. Overall, the Human APOA4 ELISA Kit provides researchers with a powerful tool for investigating the role of apolipoprotein A4 in health and disease, offering reliable and accurate measurements for a deeper understanding of its implications in metabolic disorders.
Product Name:
Human ApoA4 (Apolipoprotein A4) ELISA Kit
SKU:
HUES01668
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
2.81 ng/mL
Detection range:
4.69-300 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
