Human APOA4 Recombinant Protein (RPES1245)

SKU:
RPES1245
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
€196

Description

ELISA Kit Technical Manual

Recombinant Human APOA4 Protein (His Tag)

Background:Apolipoprotein A4 (APOA4) is a secreted protein that belongs to the apolipoprotein A1/A4/E family. Apoa-IV is a major component of HDL and chylomicrons. APOA4 is secreted into circulation on the surface of newly synthesized chylomicron particles. APOA4 play a role in the regulation of appetite and satiety in rodent models. APOA4 involved in chylomicrons and VLDL secretion and catabolism and required for efficient activation of lipoprotein lipase by ApoC-II. In addition, APOA4 is a potent activator of lecithin-cholesterol acyltransferase in vitro.
Name:Recombinant Human APOA4 Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES1245
Synonyms:Apolipoprotein A-IV; Apo-AIV; ApoA-IV; Apolipoprotein A4; APOA4
Species:Human
Expression host:Human Cells
Sequence:Glu21-Ser396
Accession:P06727
Mol Mass:44.4 kDa
AP Mol Mass:44.4 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.
UniProt Protein Details:

Protein type:Cell adhesion; Endoplasmic reticulum; Lipid-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cell surface; chylomicron; cytosol; early endosome; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space

Molecular Function:antioxidant activity; cholesterol binding; cholesterol transporter activity; copper ion binding; lipid binding; lipid transporter activity; phosphatidylcholine binding; protein binding; protein homodimerization activity

Biological Process: cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; hydrogen peroxide catabolic process; innate immune response in mucosa; leukocyte adhesion; lipid homeostasis; lipid transport; lipoprotein metabolic process; multicellular organismal lipid catabolic process; neuron projection regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; protein-lipid complex assembly; regulation of cholesterol transport; regulation of intestinal cholesterol absorption; removal of superoxide radicals; response to lipid hydroperoxide; retinoid metabolic process; reverse cholesterol transport

NCBI Summary:Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]
UniProt Code:P06727
NCBI GenInfo Identifier:93163358
NCBI Gene ID:337
NCBI Accession:P06727.3
UniProt Secondary Accession:P06727,Q14CW8, Q6Q787, A8MSL6,
UniProt Related Accession:P06727
Molecular Weight:
NCBI Full Name:Apolipoprotein A-IV
NCBI Synonym Full Names:apolipoprotein A4
NCBI Official Symbol:APOA4  
NCBI Protein Information:apolipoprotein A-IV
UniProt Protein Name:Apolipoprotein A-IV
UniProt Synonym Protein Names:Apolipoprotein A4
Protein Family:Apolipoprotein
UniProt Gene Name:APOA4  
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