The SRSF3 Monoclonal Antibody (CAB9054) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, offers exceptional performance in Western blot and immunohistochemistry applications, providing reliable and reproducible results.SRSF3, also known as SRp20, is a member of the serine/arginine-rich splicing factor family and plays a critical role in alternative splicing, mRNA export, and translation control. Dysregulation of SRSF3 has been linked to various diseases, including cancer, neurodegenerative disorders, and viral infections, making it an attractive target for research and therapeutic interventions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SRSF3 Monoclonal Antibody
SKU:
CAB9054
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1394
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SFRS3, SRp20, SRSF3
Positive Sample:
K-562, U-251MG, Mouse brain, Rat spleen
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
19kDa
Observed MW:
19kDa
The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene.
Purification Method
Affinity purification
Gene ID
6428
RRID
AB_2863648
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SRSF3 Rabbit mAb (CAB9054) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using SRSF3 Rabbit mAb (CAB9054) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using SRSF3 Rabbit mAb (CAB9054) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
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