The Assay Genie 3D Organoid Cell Viability Assay is a highly sensitive, 1-step kit to quantify the number of live viable cells directly in 2D cell culture supernatants and 3D Organoid models.This kit is an excellent choice for proliferation and cytotoxicity studies and been validated in 2D cell culture as well as 3D Apical-Out airway organoids in a high-throughput 384-well format. This technology is based on the reduction of WST-8 Tetrazolium salt by mitochondrial dehydrogenases to an orange product which is directly proportional to the number of viable cells when measured at 450nm. WST-8 is highly soluble in cell culture medium with a low cytotoxicity profile and can be added directly to cultured cells with no pre-treatments or wash steps. It is extremely powerful for high-throughput and sensitive experiments especially those requiring longer incubation steps such as 24 hour or 48 hours. This kit offers a much higher sensitivity and lower cytotoxicity than other Tetrazolium reagents such as MTS, MTT, PrestoBlue or XTT.
3D Apical-out organoids were treated with 0.1% DMSO and cell viability measured with either Assay Genie 3D Organoid Cell Viability Assay or Competitor T reagent after 1h, 3h and overnight incubations. Cell viability obtained with the 3D Organoid Cell Viability Assay on the organoids was above 100% at all time-points versus reduced viability when using PrestoBlue reagent.
3D Apical-out organoids were treated with 10% DMSO to initiate cell death and viability was measured with either Assay Genie 3D Organoid Cell Viability Assay or Competitor T reagent after 1h, 3h and overnight incubations. Cell viability obtained with the 3D Organoid Cell Viability Assay on the organoids detected reduced viability ranging from 0.5% to 5.9%.