Mouse Growth Factor Array (20 targets) (SARB0087)

SKU:
SARB0087
€597 - €1,193

Description

Mouse Growth Factor Array Product Information

Product Sku:SARB0087
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:20
Gene Symbols:AREG, CSF1, CSF2, CSF3, EGF, FGF2, FIGF, FLT1, FLT4, HGF|IGF1, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL2, IL7, KITLG, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:IGF Signaling / p53 Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Growth Factor Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Amphiregulin
  • Gene Symbol: AREG SDGF; AREGB
  • Gene ID: 11839
  • Accession #: P31955
bFGF
  • Gene Symbol: FGF2
  • Gene ID: 14173
  • Accession #: P15655
EGF
  • Gene Symbol: EGF
  • Gene ID: 13645
  • Accession #: P01132
GCSF
  • Gene Symbol: CSF3 C17orf33 GCSF
  • Gene ID: 12985
  • Accession #: P09920
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 12983
  • Accession #: P26955
HGF
  • Gene Symbol: MET
  • Gene ID: 15234
  • Accession #: Q08048
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 16000
  • Accession #: P05017
IGF-2
  • Gene Symbol: IGF2 PP1446
  • Gene ID: 16002
  • Accession #: P09535
IGFBP-2
  • Gene ID: 16008
  • Accession #: P47877
IGFBP-3
  • Gene Symbol: IGFBP3 IBP3
  • Gene ID: 16009
  • Accession #: P47878
IGFBP-5
  • Gene Symbol: IGFBP5 IBP5
  • Gene ID: 16011
  • Accession #: Q07079
IGFBP-6
  • Gene Symbol: IGFBP6 IBP6
  • Gene ID: 16012
  • Accession #: P47880
IL-2
  • Gene Symbol: IL2 IL-2
  • Gene ID: 16183
  • Accession #: P04351
  

Mouse Growth Factor Array Kit Components

  • Mouse Growth Factor Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Growth Factor Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Amphiregulin
Target:
bFGF
Target:
EGF
Target:
GCSF
Target:
GM-CSF
Target:
HGF
Target:
IGF-1
Target:
IGF-2
Target:
IGFBP-2
Target:
IGFBP-3
Target:
IGFBP-5
Target:
IGFBP-6
Target:
IL-2
Target:
IL-7
Target:
M-CSF
Target:
SCF
Target:
VEGF-A
Target:
VEGF-D
Target:
VEGFR1
Target:
VEGFR3
Reactivity:
Mouse
Application:
Multiplex Array
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