Human Growth Factor Array (41 targets) (SARB0045)

SKU:
SARB0045
€644 - €1,297

Description

Human Growth Factor Array Product Information

Product Sku:SARB0045
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:41
Gene Symbols:AREG, CSF1, CSF1R, CSF2, CSF3, EGF, EGFR, FGF2, FGF4, FGF6|FGF7, FIGF, FLT4, GDNF, HBEGF, HGF, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP6, KDR, KIT, KITLG, NGF, NTF3, NTF4, PDGFA, PDGFB, PDGFRA, PDGFRB, PGF, TGFA, TGFB1, TGFB2, TGFB3, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Growth Factor Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

AmphiregulinbFGFbeta-NGFEGFEGFR
FGF-4FGF-6FGF-7 (KGF)GCSFGDNF
GM-CSFHB-EGFHGFIGFBP-1IGFBP-2
IGFBP-3IGFBP-4IGFBP-6IGF-1IGF-1 R
IGF-2M-CSFM-CSF RNT-3NT-4
PDGF R alphaPDGF R betaPDGF-AAPDGF-ABPDGF-BB
PLGFSCFSCF R (CD117/c-kit)TGF alphaTGF beta 1
TGF beta 2TGF beta 3VEGF-AVEGFR2VEGFR3
VEGF-D    

Human Growth Factor Array Kit Components

  • Human Growth Factor Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Growth Factor Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
View AllClose

Additional Information

Product type:
Protein Array
Target:
Amphiregulin
Target:
bFGF
Target:
beta-NGF
Target:
EGF
Target:
EGFR
Target:
FGF-4
Target:
FGF-6
Target:
FGF-7 (KGF)
Target:
GCSF
Target:
GDNF
Target:
GM-CSF
Target:
HB-EGF
Target:
HGF
Target:
IGFBP-1
Target:
IGFBP-2
Target:
IGFBP-3
Target:
IGFBP-4
Target:
IGFBP-6
Target:
IGF-1
Target:
IGF-1 R
Target:
IGF-2
Target:
M-CSF
Target:
M-CSF R
Target:
NT-3
Target:
NT-4
Target:
PDGF R alpha
Target:
PDGF R beta
Target:
PDGF-AA
Target:
PDGF-AB
Target:
PDGF-BB
Target:
PLGF
Target:
SCF
Target:
SCF R (CD117/c-kit)
Target:
TGF alpha
Target:
TGF beta 1
Target:
TGF beta 2
Target:
TGF beta 3
Target:
VEGF-A
Target:
VEGFR2
Target:
VEGFR3
Target:
VEGF-D
Reactivity:
Human
Application:
Multiplex Array
View AllClose