Description
Human Inflammation Array (20 targets) (SARB0050)
The Human Inflammation Array (SAR-B0050) from Assay Genie is a powerful tool for studying inflammation-related proteins in human samples. This array allows for the simultaneous detection of 20 different targets involved in the inflammatory response, providing valuable insight into the complex signaling pathways regulating inflammation.Researchers can investigate the levels of cytokines, chemokines, growth factors, and other proteins involved in inflammation using this array. By measuring multiple targets at once, researchers can gain a more comprehensive understanding of the inflammatory process and identify potential therapeutic targets for conditions such as autoimmune disorders, cancer, and chronic inflammatory diseases.
The Human Inflammation Array is easy to use and provides accurate and reliable results. It is suitable for a wide range of sample types, including serum, plasma, and cell lysates. With its high sensitivity and specificity, this array is an essential tool for researchers studying inflammation and its role in human health and disease.
| Product Sku: | SARB0050 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 20 |
| Gene Symbols: | CCL1, CCL11, CCL24, CSF2, CSF3, CXCL8, IFNG, IL10, IL11, IL12A|IL13, IL1A, IL1B, IL2, IL3, IL4, IL6, IL7, TIMP2 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| GCSF
| GM-CSF
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
|
IL-6
| IL-7
| IL-8 (CXCL8)
| IL-10
| IL-11
|
IL-12 p40
| IL-12 p70
| IL-13
| I-309 (TCA-3/CCL1)
| TIMP-2
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Inflammation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
