Human Inflammation Array (20 targets) (SARB0051)

SKU:
SARB0051
€531 - €1,047

Description

Human Inflammation Array Product Information

Product Sku:SARB0051
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:20
Gene Symbols:CCL15, CCL2, CCL3, CCL4, CCL5, CCL8, CSF1, CXCL10, CXCL9, ICAM1|IL15, IL16, IL17A, IL6R, LTA, PDGFB, TGFB1, TNF, TNFRSF1A, TNFRSF1B
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:MAPK Signaling / NFkB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Inflammation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

ICAM-1 (CD54)
  • Gene Symbol: ICAM1
  • Gene ID: 3383
  • Accession #: P05362
IL-6 R
  • Gene Symbol: IL6R
  • Gene ID: 3570
  • Accession #: P08887
IL-15
  • Gene Symbol: IL15
  • Gene ID: 3600
  • Accession #: P40933
IL-16
  • Gene Symbol: IL16
  • Gene ID: 3603
  • Accession #: Q14005
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IP-10 (CXCL10)
  • Gene Symbol: CXCL10
  • Gene ID: 3627
  • Accession #: P02778
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
MCP-2 (CCL8)
  • Gene Symbol: CCL8
  • Gene ID: 6355
  • Accession #: P80075
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
MIG (CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: 4283
  • Accession #: Q07325
MIP-1 alpha (CCL3)
  • Gene Symbol: CCL3
  • Gene ID: 6348
  • Accession #: P10147
MIP-1 beta (CCL4)
  • Gene Symbol: CCL4
  • Gene ID: 6351
  • Accession #: P13236
MIP-1 delta (CCL15)
  • Gene Symbol: CCL15
  • Gene ID: 6359
  • Accession #: Q16663
  

Human Inflammation Array Kit Components

  • Human Inflammation Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Inflammation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
ICAM-1 (CD54)
Target:
IL-6 R
Target:
IL-15
Target:
IL-16
Target:
IL-17A
Target:
IP-10 (CXCL10)
Target:
MCP-1 (CCL2)
Target:
MCP-2 (CCL8)
Target:
M-CSF
Target:
MIG (CXCL9)
Target:
MIP-1 alpha (CCL3)
Target:
MIP-1 beta (CCL4)
Target:
MIP-1 delta (CCL15)
Target:
RANTES (CCL5)
Target:
TGF beta 1
Target:
TNF alpha
Target:
TNF beta (TNFSF1B)
Target:
TNF RI (TNFRSF1A)
Target:
TNF RII (TNFRSF1B)
Target:
PDGF-BB
Reactivity:
Human
Application:
Multiplex Array
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