Human Inflammation Array (40 targets) (SARB0052)

SKU:
SARB0052
€644 - €1,297

Description

Human Inflammation Array Product Information

Product Sku:SARB0052
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:40
Gene Symbols:CCL1, CCL11, CCL15, CCL2, CCL24, CCL3, CCL4, CCL5, CCL8, CSF1|CSF2, CSF3, CXCL10, CXCL8, CXCL9, ICAM1, IFNG, IL10, IL11, IL12A, IL12B, IL13, IL15, IL16, IL17A, IL1A, IL1B, IL2, IL3, IL4, IL6, IL6R, IL7, LTA, PDGFB, TGFB1, TIMP2, TNF, TNFRSF1A, TNFRSF1B
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Inflammation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Eotaxin-1 (CCL11)Eotaxin-2 (MPIF-2/CCL24)GCSFGM-CSFICAM-1 (CD54)
IFN-gammaI-309 (TCA-3/CCL1)IL-1 alpha (IL-1 F1)IL-1 beta (IL-1 F2)IL-2
IL-3IL-4IL-6IL-6 RIL-7
IL-8 (CXCL8)IL-10IL-11IL-12 p40IL-12 p70
IL-13IL-15IL-16IL-17AIP-10 (CXCL10)
MCP-1 (CCL2)MCP-2 (CCL8)M-CSFMIG (CXCL9)MIP-1 alpha (CCL3)
MIP-1 beta (CCL4)MIP-1 delta (CCL15)RANTES (CCL5)TGF beta 1TNF alpha
TNF beta (TNFSF1B)TNF RI (TNFRSF1A)TNF RII (TNFRSF1B)PDGF-BBTIMP-2

Human Inflammation Array Kit Components

  • Human Inflammation Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Inflammation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
View AllClose

Additional Information

Product type:
Protein Array
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
GCSF
Target:
GM-CSF
Target:
ICAM-1 (CD54)
Target:
IFN-gamma
Target:
I-309 (TCA-3/CCL1)
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-2
Target:
IL-3
Target:
IL-4
Target:
IL-6
Target:
IL-6 R
Target:
IL-7
Target:
IL-8 (CXCL8)
Target:
IL-10
Target:
IL-11
Target:
IL-12 p40
Target:
IL-12 p70
Target:
IL-13
Target:
IL-15
Target:
IL-16
Target:
IL-17A
Target:
IP-10 (CXCL10)
Target:
MCP-1 (CCL2)
Target:
MCP-2 (CCL8)
Target:
M-CSF
Target:
MIG (CXCL9)
Target:
MIP-1 alpha (CCL3)
Target:
MIP-1 beta (CCL4)
Target:
MIP-1 delta (CCL15)
Target:
RANTES (CCL5)
Target:
TGF beta 1
Target:
TNF alpha
Target:
TNF beta (TNFSF1B)
Target:
TNF RI (TNFRSF1A)
Target:
TNF RII (TNFRSF1B)
Target:
PDGF-BB
Target:
TIMP-2
Reactivity:
Human
Application:
Multiplex Array
View AllClose