Canine Inflammation Array (20 targets) (SARB0010)

SKU:
SARB0010
€596 - €1,173

Description

Canine Inflammation Array Product Information

Product Sku:SARB0010
Size:2, 4, or 8
Species Detected:Canine
Number of Targets Detected:20
Gene Symbols:CCL2, CCL4, CSF2, CXCL8, IFNG, IL10, IL12B, IL17A, IL1B, IL6|TNF, TNFRSF1A
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Canine Inflammation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 403923
  • Accession #: P48749
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 403801
  • Accession #: P42161
IL-1 alpha (IL-1 F1)IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID:
  • Accession #: Q28292
IL-1 Ra (IL-1 F3)
IL-10
  • Gene Symbol: IL10
  • Gene ID: 403628
  • Accession #: P48411
IL-12 p40
  • Gene Symbol: IL12B
  • Gene ID: 403976
  • Accession #: Q28268
IL-13IL-17A
  • Gene Symbol: IL-17A IL17A
  • Gene ID: 481837
  • Accession #: C6L8D7
IL-21
IL-6
  • Gene Symbol: IL6
  • Gene ID: 403985
  • Accession #: P41323
IL-8 (CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 403850
  • Accession #: P41324
MCP-1 (CCL2)
  • Gene Symbol: CCL2 MCP1 SCYA2
  • Gene ID: 403981
  • Accession #: P52203

Canine Inflammation Array Kit Components

  • Canine Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Canine Inflammation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
GM-CSF
Target:
IFN-gamma
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-1 Ra (IL-1 F3)
Target:
IL-10
Target:
IL-12 p40
Target:
IL-13
Target:
IL-17A
Target:
IL-21
Target:
IL-6
Target:
IL-8 (CXCL8)
Target:
MCP-1 (CCL2)
Target:
MIP-1 beta (CCL4)
Target:
RAGE
Target:
RANTES (CCL5)
Target:
Resistin
Target:
TNF alpha
Target:
TNF RI (TNFRSF1A)
Target:
Trappin-2
Reactivity:
Canine
Application:
Multiplex Array
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