Description
Bovine Inflammation Array (16 targets) (SARB0005)
The Bovine Inflammation Array (SARB0005) from Assay Genie is a powerful tool for researchers studying inflammation in bovine samples. This array allows for the simultaneous detection of 16 different targets related to inflammation, providing comprehensive insight into the inflammatory response in bovine species.With targets including cytokines, chemokines, and growth factors, the Bovine Inflammation Array offers a high-throughput and cost-effective solution for profiling inflammatory markers in bovine samples. The array is designed for use with a variety of sample types, including serum, plasma, and cell culture supernatants, making it versatile for a wide range of experimental settings.
By utilizing the Bovine Inflammation Array, researchers can gain a deeper understanding of the inflammatory processes at play in bovine samples, leading to new insights into disease mechanisms, treatment strategies, and biomarker discovery. This array is an essential tool for any researcher interested in studying inflammation in bovine species.
| Product Sku: | SARB0005 |
| Size: | 2, 4, or 8 |
| Species Detected: | Bovine |
| Number of Targets Detected: | 16 |
| Gene Symbols: | CCL2, CXCL10, CXCL9, IFNG, IL10, IL13, IL15, IL17A, IL1A, IL1B|IL21, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
IFN alpha
| IFN-beta
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-10
| IL-13
| IL-15
| IL-17A
| IL-21
|
IP-10 (CXCL10)
| MCP-1 (CCL2)
| MIG (CXCL9)
| MIP-1 beta (CCL4)
| RANTES (CCL5) |
TNF alpha
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Bovine Inflammation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
