Mouse Inflammation Array (40 targets) (SARB0090)

SKU:
SARB0090
€543 - €1,075

Description

Mouse Inflammation Array Product Information

Product Sku:SARB0090
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:40
Gene Symbols:CCL1, CCL11, CCL2, CCL24, CCL25, CCL3, CCL5, CCL9, CSF1, CSF2|CSF3, CX3CL1, CXCL1, CXCL11, CXCL12, CXCL13, CXCL5, CXCL9, FASLG, IFNG, IL10, IL12A, IL13, IL17A, IL1A, IL1B, IL2, IL3, IL4, IL6, IL9, LEP, TIMP1, TIMP2, TNF, TNFRSF1A, TNFRSF1B, TNFSF8, XCL1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Inflammation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

BLC (CXCL13)CD30 Ligand (TNFSF8)Eotaxin-1 (CCL11)Eotaxin-2 (MPIF-2/CCL24)Fas Ligand (TNFSF6)
Fractalkine (CX3CL1)GCSFGM-CSFIFN-gammaIL-1 alpha (IL-1 F1)
IL-1 beta (IL-1 F2)IL-2IL-3IL-4IL-6
IL-9IL-10IL-12 p40/p70IL12BIL-12 p70
IL-13IL-17AI-TAC (CXCL11)KC (CXCL1)Leptin
LIXLymphotactin (XCL1)MCP-1 (CCL2)M-CSFMIG (CXCL9)
MIP-1 alpha (CCL3)MIP-1 gammaRANTES (CCL5)SDF-1 alpha (CXCL12 alpha)I-309 (TCA-3/CCL1)
TECK (CCL25)TIMP-1TIMP-2TNF alphaTNF RI (TNFRSF1A)
TNF RII (TNFRSF1B)    

Mouse Inflammation Array Kit Components

  • Mouse Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Inflammation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
BLC (CXCL13)
Target:
CD30 Ligand (TNFSF8)
Target:
Eotaxin-1 (CCL11)
Target:
Eotaxin-2 (MPIF-2/CCL24)
Target:
Fas Ligand (TNFSF6)
Target:
Fractalkine (CX3CL1)
Target:
GCSF
Target:
GM-CSF
Target:
IFN-gamma
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-2
Target:
IL-3
Target:
IL-4
Target:
IL-6
Target:
IL-9
Target:
IL-10
Target:
IL-12 p40/p70
Target:
IL-12 p70
Target:
IL-13
Target:
IL-17A
Target:
I-TAC (CXCL11)
Target:
KC (CXCL1)
Target:
Leptin
Target:
LIX
Target:
Lymphotactin (XCL1)
Target:
MCP-1 (CCL2)
Target:
M-CSF
Target:
MIG (CXCL9)
Target:
MIP-1 alpha (CCL3)
Target:
MIP-1 gamma
Target:
RANTES (CCL5)
Target:
SDF-1 alpha (CXCL12 alpha)
Target:
I-309 (TCA-3/CCL1)
Target:
TECK (CCL25)
Target:
TIMP-1
Target:
TIMP-2
Target:
TNF alpha
Target:
TNF RI (TNFRSF1A)
Target:
TNF RII (TNFRSF1B)
Reactivity:
Mouse
Application:
Multiplex Array
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