Porcine Inflammation Array (26 targets) (SARB0097)

SKU:
SARB0097
€618 - €1,220

Description

Porcine Inflammation Array Product Information

Product Sku:SARB0097
Size:2, 4, or 8
Species Detected:Porcine
Number of Targets Detected:26
Gene Symbols:CCL4, CCL5, CSF2, CXCL8, CXCL9, IFN-BETA, IFNB, IFNB1, IFNG, IGIF|IL10, IL12A, IL13, IL15, IL17, IL17A, IL17F, IL18, IL1A, IL1B, IL1RN, IL21, IL22, IL28B, IL4, IL6, IL8, INTERFERON-BETA-1, INTERFERON-BETA/POIFN-BETA, IRAP1, MIF, MIP1B, SCY4A, SCYA5, TNF, TNFA, TNFSF2
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Porcine Inflammation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Eotaxin-1 (CCL11)
  • Accession #:
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 397208
  • Accession #: Q29118
IFN alpha
  • Gene ID:
  • Accession #: P49879
IFN-beta
  • Gene Symbol: interferon-beta-1 IFN-beta IFNB IFNB1 interferon-beta/ poIFN-beta
  • Accession #: Q29412
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 396991
  • Accession #: P17803
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 397094
  • Accession #: P18430
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 397122
  • Accession #: P26889
IL-1 Ra (IL-1 F3)
  • Gene Symbol: IL1RN IRAP1
  • Gene ID: 397499
  • Accession #: Q29056
IL-10
  • Gene Symbol: IL10
  • Gene ID: 397106
  • Accession #: Q29055
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 397053
  • Accession #: Q29053
IL-13
  • Gene Symbol: IL13
  • Gene ID: 396721
  • Accession #: Q95J68
IL-15
  • Gene Symbol: IL15
  • Gene ID: 397683
  • Accession #: Q95253
IL-17A
  • Gene Symbol: IL17A IL17
  • Gene ID: 449530
  • Accession #: Q60I29
  

Porcine Inflammation Array Kit Components

  • Porcine Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Porcine Inflammation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Eotaxin-1 (CCL11)
Target:
GM-CSF
Target:
IFN alpha
Target:
IFN-beta
Target:
IFN-gamma
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-1 Ra (IL-1 F3)
Target:
IL-10
Target:
IL-12 p70
Target:
IL-13
Target:
IL-15
Target:
IL-17A
Target:
IL-17F
Target:
IL-18
Target:
IL-21
Target:
IL-22
Target:
IL-28B
Target:
IL-4
Target:
IL-6
Target:
IL-8 (CXCL8)
Target:
MIF
Target:
MIG (CXCL9)
Target:
MIP-1 beta (CCL4)
Target:
RANTES (CCL5)
Target:
TNF alpha
Reactivity:
Porcine
Application:
Multiplex Array
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