Human Apoptosis Array (43 targets) (SARB0021)

SKU:
SARB0021
€552 - €1,094

Description

Human Apoptosis Array Product Information

Product Sku:SARB0021
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:43
Gene Symbols:BAD, BAX, BCL2, BCL2L11, BCL2L2, BID, BIRC3, BIRC5, BIRC7, CASP3|CASP8, CD40, CD40LG, CDKN1A, CYCS, DIABLO, FAS, FASLG, HSPA14, HSPA4, HSPB1, HTRA2, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6, LTA, SSSCA1, TNF, TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, TNFRSF1A, TNFRSF1B, TNFRSF21, TP53, XIAP
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / IGF Signaling / p53 Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Apoptosis Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

badbaxbcl-2bcl-wBID
BIMCaspase-3Caspase-8CD40 (TNFRSF5)CD40 Ligand (TNFSF5)
cIAP-2Cytochrome CDR6 (TNFRSF21)Fas (TNFRSF6/Apo-1)Fas Ligand (TNFSF6)
HSP27HSP60HSP70HTRA2IGF-1
IGF-2IGFBP-1IGFBP-2IGFBP-3IGFBP-4
IGFBP-5IGFBP-6IGF-1 Rlivinp21
p27p53SMACSurvivin (BIRC5)TNF RI (TNFRSF1A)
TNF RII (TNFRSF1B)TNF alphaTNF beta (TNFSF1B)TRAIL R1 (TNFRSF10A/DR4)TRAIL R2 (TNFRSF10B/DR5)
TRAIL R3 (TNFRSF10C)TRAIL R4 (TNFRSF10D)XIAP  

Human Apoptosis Array Kit Components

  • Human Apoptosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Apoptosis Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
bad
Target:
bax
Target:
bcl-2
Target:
bcl-w
Target:
BID
Target:
BIM
Target:
Caspase-3
Target:
Caspase-8
Target:
CD40 (TNFRSF5)
Target:
CD40 Ligand (TNFSF5)
Target:
cIAP-2
Target:
Cytochrome C
Target:
DR6 (TNFRSF21)
Target:
Fas (TNFRSF6/Apo-1)
Target:
Fas Ligand (TNFSF6)
Target:
HSP27
Target:
HSP60
Target:
HSP70
Target:
HTRA2
Target:
IGF-1
Target:
IGF-2
Target:
IGFBP-1
Target:
IGFBP-2
Target:
IGFBP-3
Target:
IGFBP-4
Target:
IGFBP-5
Target:
IGFBP-6
Target:
IGF-1 R
Target:
livin
Target:
p21
Target:
p27
Target:
p53
Target:
SMAC
Target:
Survivin (BIRC5)
Target:
TNF RI (TNFRSF1A)
Target:
TNF RII (TNFRSF1B)
Target:
TNF alpha
Target:
TNF beta (TNFSF1B)
Target:
TRAIL R1 (TNFRSF10A/DR4)
Target:
TRAIL R2 (TNFRSF10B/DR5)
Target:
TRAIL R3 (TNFRSF10C)
Target:
TRAIL R4 (TNFRSF10D)
Target:
XIAP
Reactivity:
Human
Application:
Multiplex Array
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