Mouse Apoptosis Signaling Pathway Array (17 targets) (SARB0074)

SKU:
SARB0074
€562 - €1,115

Description

Mouse Apoptosis Signaling Pathway Array Product Information

Product Sku:SARB0074
Size:2, 4, or 8
Species Detected:Mouse
Number of Targets Detected:17
Gene Symbols:AKT1, ATM, BAD, CASP3, CASP7, CDKN1B, CHEK1, EIF2S1, HSPB1, MAP3K7|MAPK14, MAPK8, NFKBIA, RELA, SMAD2, TP53
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / HER/ErbB Signaling / HIF-1 alpha Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PI3K-AKT Signaling / PKC Signaling / TGF-beta Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Mouse Apoptosis Signaling Pathway Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

AKT (P-Ser473)
  • Gene Symbol: Akt1 Akt Rac
  • Gene ID: 11651
  • Accession #: P31750
ATM (P-Ser1981)
  • Gene Symbol: Atm
  • Gene ID: 11920
  • Accession #: Q62388
BAD (P-Ser112)
  • Gene Symbol: Bad Bbc6
  • Gene ID: 12015
  • Accession #: Q61337
Caspase-3 (P-Asp175)
  • Gene Symbol: Casp3 Cpp32
  • Gene ID: 12367
  • Accession #: P70677
Caspase-7 (P-Asp198)
  • Gene Symbol: Casp7 Lice2 Mch3
  • Gene ID: 12369
  • Accession #: P97864
CHK1 (P-Ser296)
  • Gene Symbol: Chek1 Chk1
  • Gene ID: 12649
  • Accession #: O35280
eIF-2a (P-Ser52)
  • Gene Symbol: Eif2s1 Eif2a
  • Gene ID: 13665
  • Accession #: Q6ZWX6
ERK1/2 (P-Thr202)
  • Accession #: Q63844
  • P63085
  • HSP27 (P-Ser82)
  • Gene Symbol: Hspb1 Hsp25 Hsp27
Gene ID: 15507
  • Accession #: P14602
  • IKBa (P-Ser32)
  • Gene Symbol: Nfkbia Ikba
Gene ID: 18035
  • Accession #: Q9Z1E3
  • JNK (P-Thr183)
  • Gene Symbol: Mapk8 Jnk1 Prkm8
Gene ID: 26419
  • Accession #: Q91Y86
  • NF-kB P65 (P-Ser536)
  • Gene Symbol: Rela Nfkb3
Gene ID: 19697
  • Accession #: Q04207
  • p27 (P-Thr198)
  • Gene Symbol: Cdkn1b
  

Mouse Apoptosis Signaling Pathway Array Kit Components

  • Mouse Apoptosis Signaling Pathway Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Mouse Apoptosis Signaling Pathway Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
AKT (P-Ser473)
Target:
ATM (P-Ser1981)
Target:
BAD (P-Ser112)
Target:
Caspase-3 (P-Asp175)
Target:
Caspase-7 (P-Asp198)
Target:
CHK1 (P-Ser296)
Target:
eIF-2a (P-Ser52)
Target:
ERK1/2 (P-Thr202)
Target:
P63085
Target:
HSP27 (P-Ser82)
Target:
IKBa (P-Ser32)
Target:
JNK (P-Thr183)
Target:
NF-kB P65 (P-Ser536)
Target:
p27 (P-Thr198)
Target:
p38 (P-Thr180/Tyr182)
Target:
P53 (P-Ser15)
Target:
SMAD2 (P-Ser245)
Target:
TAK1 (P-Ser412)
Reactivity:
Mouse
Application:
Multiplex Array
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