Human/Mouse AKT Pathway Phosphorylation Array (18 targets) (SARB0017)

SKU:
SARB0017
€523 - €1,030

Description

Human/Mouse AKT Pathway Phosphorylation Array Product Information

Product Sku:SARB0017
Size:2, 4, or 8
Species Detected:Human / Mouse
Number of Targets Detected:18
Gene Symbols:AKT1, AKT1S1, BAD, CDKN1B, EIF4EBP1, GSK3A, GSK3B, MAPK1, MAPK3, MTOR|PDK1, PRKAA1, PTEN, RAF1, RPS6, RPS6KA1, RPS6KA3, RPS6KB1, TP53
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / AMPK Signaling / HER/ErbB Signaling / HIF-1 alpha Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / mTOR Signaling / PI3K-AKT Signaling / TGF-beta Signaling / Wnt/beta-Catenin Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human/Mouse AKT Pathway Phosphorylation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Akt (P-Ser473) (PKB (P-Ser473))
  • Gene Symbol: AKT1 PKB RAC
  • Gene ID: 207
  • Accession #: P31749
AMPKa (P-Thr172)
  • Gene Symbol: PRKAA1 AMPK1
  • Gene ID: 5562
  • Accession #: Q13131
BAD (P-Ser112)
  • Gene Symbol: BAD BBC6 BCL2L8
  • Gene ID: 572
  • Accession #: Q92934
4E-BP1 (P-Thr36)
  • Gene Symbol: EIF4EBP1
  • Gene ID: 1978
  • Accession #: Q13541
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
  • Gene Symbol: MAPK1 ERK2 PRKM1 PRKM2
  • Gene ID: 5594
  • Accession #: P28482
GSK3a (P-Ser21)
  • Gene Symbol: GSK3A
  • Gene ID: 2931
  • Accession #: P49840
GSK3b (P-Ser9)
  • Gene Symbol: GSK3B
  • Gene ID: 2932
  • Accession #: P49841
mTOR (P-Ser2448)
  • Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1
  • Gene ID: 2475
  • Accession #: P42345
p27 (P-Thr198)
  • Gene Symbol: PSMD9
  • Gene ID: 5715
  • Accession #: O00233
P53 (P-Ser15)
  • Gene Symbol: TP53 P53
  • Gene ID: 6198
  • Accession #: P23443
P70S6K (P-Thr421/Ser424)
  • Gene Symbol: RPS6KB1 STK14A
  • Gene ID: 6198
  • Accession #: P23443
PDK1 (P-Ser241)
  • Gene Symbol: PDK1 PDHK1
  • Gene ID: 5170
  • Accession #: O15530
PRAS40 (P-Thr246)
  • Gene Symbol: AKT1S1 PRAS40
  • Gene ID: 84335
  • Accession #: Q96B36
  

Human/Mouse AKT Pathway Phosphorylation Array Kit Components

  • Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human/Mouse AKT Pathway Phosphorylation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Phosphorylation Array
Target:
Akt (P-Ser473) (PKB (P-Ser473))
Target:
AMPKa (P-Thr172)
Target:
BAD (P-Ser112)
Target:
4E-BP1 (P-Thr36)
Target:
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
Target:
GSK3a (P-Ser21)
Target:
GSK3b (P-Ser9)
Target:
mTOR (P-Ser2448)
Target:
p27 (P-Thr198)
Target:
P53 (P-Ser15)
Target:
P70S6K (P-Thr421/Ser424)
Target:
PDK1 (P-Ser241)
Target:
PRAS40 (P-Thr246)
Target:
PTEN (P-Ser380)
Target:
Raf-1 (Ser301)
Target:
RPS6 (P-Ser235/Ser236)
Target:
RSK1 (P-Ser380)
Target:
RSK2 (P-Ser386)
Reactivity:
Human
Reactivity:
Mouse
Application:
Multiplex Array
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