Human/Mouse AKT Pathway Phosphorylation Array (18 targets) (SARB0017)
- SKU:
- SARB0017
- Product Type:
- Phosphorylation Array
- Analytes:
- Akt (P-Ser473) (PKB (P-Ser473))
- AMPKa (P-Thr172)
- BAD (P-Ser112)
- 4E-BP1 (P-Thr36)
- ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
- GSK3a (P-Ser21)
- GSK3b (P-Ser9)
- mTOR (P-Ser2448)
- p27 (P-Thr198)
- P53 (P-Ser15)
- P70S6K (P-Thr421/Ser424)
- PDK1 (P-Ser241)
- PRAS40 (P-Thr246)
- PTEN (P-Ser380)
- Raf-1 (Ser301)
- RPS6 (P-Ser235/Ser236)
- RSK1 (P-Ser380)
- RSK2 (P-Ser386)
- Reactivity:
- Human
- Mouse
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
Product Sku: | SARB0017 |
Size: | 2, 4, or 8 |
Species Detected: | Human / Mouse |
Number of Targets Detected: | 18 |
Gene Symbols: | AKT1, AKT1S1, BAD, CDKN1B, EIF4EBP1, GSK3A, GSK3B, MAPK1, MAPK3, MTOR|PDK1, PRKAA1, PTEN, RAF1, RPS6, RPS6KA1, RPS6KA3, RPS6KB1, TP53 |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | Akt Signaling / AMPK Signaling / HER/ErbB Signaling / HIF-1 alpha Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / mTOR Signaling / PI3K-AKT Signaling / TGF-beta Signaling / Wnt/beta-Catenin Signaling |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
Akt (P-Ser473) (PKB (P-Ser473))
| AMPKa (P-Thr172)
| BAD (P-Ser112)
| 4E-BP1 (P-Thr36)
| ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
|
GSK3a (P-Ser21)
| GSK3b (P-Ser9)
| mTOR (P-Ser2448)
| p27 (P-Thr198)
| P53 (P-Ser15)
|
P70S6K (P-Thr421/Ser424)
| PDK1 (P-Ser241)
| PRAS40 (P-Thr246)
| PTEN (P-Ser380)
| Raf-1 (Ser301)
|
RPS6 (P-Ser235/Ser236)
| RSK1 (P-Ser380)
| RSK2 (P-Ser386)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated anti-IgG |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |