Description
Human TGF beta Pathway Phosphorylation Array (8 targets) (SARB0069)
The Human TGF-beta Pathway Phosphorylation Array (SARB0069) from AssayGenie is a powerful tool for studying the TGF-beta signaling pathway in human samples. This array allows for the simultaneous detection and analysis of phosphorylation levels of 8 key targets involved in the TGF-beta pathway. With high sensitivity and specificity, this array provides researchers with valuable information on the activation status of key signaling molecules in response to TGF-beta stimulation. It is ideal for studies in cell signaling, cancer biology, and drug development related to TGF-beta signaling.By enabling multiplex analysis of key phosphorylation events in the TGF-beta pathway, this array allows for a comprehensive understanding of the signaling cascade and its impact on cellular processes.
Researchers can use this tool to uncover new insights into the role of TGF-beta signaling in health and disease, ultimately driving advancements in therapeutic strategies targeting this pathway.Overall, the Human TGF-beta Pathway Phosphorylation Array (SARB0069) offers a reliable and efficient solution for investigating TGF-beta signaling dynamics in human samples, providing a valuable resource for researchers in the fields of molecular biology, cancer research, and drug discovery.
| Product Sku: | SARB0069 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 8 |
| Gene Symbols: | ATF2, FOS, JUN, MAP3K7, SMAD1, SMAD2, SMAD4, SMAD5 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | PKC Signaling / TGF-beta Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
c-Jun (P-Ser73)
| SMAD1 (P-Ser463/465)
| SMAD5 (P-Ser463/465)
| SMAD4 (P-Thr277)
| ATF2 (P-Thr69/71)
|
C-Fos (P-Thr232)
| SMAD2 (P-Ser245/250/255)
| TAK1 (P-Ser412)
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human TGF beta Phosphorylation Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 500X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
