Description
Human JAK/STAT Pathway Phosphorylation Array (12 targets) (SARB0054)
The Human JAK-STAT Pathway Phosphorylation Array (SARB0054) is a powerful tool for researchers studying signal transduction pathways in immune regulation and inflammation. This array allows for the simultaneous detection and analysis of phosphorylation levels of 12 key targets involved in the JAK-STAT signaling pathway, including STAT proteins and various kinases.With the ability to assess the activation status of multiple targets at once, researchers can gain valuable insights into the intricate mechanisms underlying immune responses and cellular signaling. The array is compatible with human samples and is optimized for sensitivity and specificity, providing reliable and reproducible results.
By utilizing the Human JAK-STAT Pathway Phosphorylation Array, researchers can streamline their studies on immune signaling pathways and gain a deeper understanding of how dysregulation in these pathways contributes to diseases such as cancer, autoimmune disorders, and inflammatory conditions. This innovative tool offers a comprehensive and efficient approach to investigating the complex network of signaling events that govern immune response modulation.
| Product Sku: | SARB0054 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 12 |
| Gene Symbols: | EGFR, JAK1, JAK2, PTPN11, PTPN6, SRC, STAT1, STAT2, STAT3, STAT5A|STAT6, TYK2 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / HER/ErbB Signaling / JAK/STAT Signaling / MAPK Signaling / Notch Signaling / PI3K-AKT Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
EGFR (P-Ser1070)
| JAK1 (P-Tyr1022)
| JAK2 (P-Tyr1007/1008)
| SHP-1 (P-Ser591)
| SHP2 (P-Tyr542)
|
Src (P-Tyr419)
| STAT1 (P-Ser727)
| STAT2 (P-Tyr689)
| STAT3 (P-Tyr705)
| STAT5 (P-Tyr694)
|
STAT6 (P-Tyr641)
| TYK2 (P-Tyr1054)
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human JAK/STAT Pathway Phosporylation Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
