Human JAK/STAT Pathway Phosphorylation Array (12 targets) (SARB0054)
- SKU:
- SARB0054
- Product Type:
- Phosphorylation Array
- Analytes:
- EGFR (P-Ser1070)
- JAK1 (P-Tyr1022)
- JAK2 (P-Tyr1007/1008)
- SHP-1 (P-Ser591)
- SHP2 (P-Tyr542)
- Src (P-Tyr419)
- STAT1 (P-Ser727)
- STAT2 (P-Tyr689)
- STAT3 (P-Tyr705)
- STAT5 (P-Tyr694)
- STAT6 (P-Tyr641)
- TYK2 (P-Tyr1054)
- Reactivity:
- Human
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
Product Sku: | SARB0054 |
Size: | 2, 4, or 8 |
Species Detected: | Human |
Number of Targets Detected: | 12 |
Gene Symbols: | EGFR, JAK1, JAK2, PTPN11, PTPN6, SRC, STAT1, STAT2, STAT3, STAT5A|STAT6, TYK2 |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | Akt Signaling / HER/ErbB Signaling / JAK/STAT Signaling / MAPK Signaling / Notch Signaling / PI3K-AKT Signaling |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
EGFR (P-Ser1070)
| JAK1 (P-Tyr1022)
| JAK2 (P-Tyr1007/1008)
| SHP-1 (P-Ser591)
| SHP2 (P-Tyr542)
|
Src (P-Tyr419)
| STAT1 (P-Ser727)
| STAT2 (P-Tyr689)
| STAT3 (P-Tyr705)
| STAT5 (P-Tyr694)
|
STAT6 (P-Tyr641)
| TYK2 (P-Tyr1054)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human JAK/STAT Pathway Phosporylation Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated anti-IgG |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |