Human JAK/STAT Pathway Phosphorylation Array (12 targets) (SARB0054)

SKU:
SARB0054
€496 - €971

Description

Human JAK/STAT Pathway Phosphorylation Array Product Information

Product Sku:SARB0054
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:12
Gene Symbols:EGFR, JAK1, JAK2, PTPN11, PTPN6, SRC, STAT1, STAT2, STAT3, STAT5A|STAT6, TYK2
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / HER/ErbB Signaling / JAK/STAT Signaling / MAPK Signaling / Notch Signaling / PI3K-AKT Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human JAK/STAT Pathway Phosphorylation Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

EGFR (P-Ser1070)
  • Gene Symbol: EGFR ERBB ERBB1 HER1
  • Gene ID: 1956
  • Accession #: P00533
JAK1 (P-Tyr1022)
  • Gene Symbol: JAK1 JAK1A JAK1B
  • Gene ID: 3716
  • Accession #: P23458
JAK2 (P-Tyr1007/1008)
  • Gene Symbol: JAK2
  • Gene ID: 3717
  • Accession #: O60674
SHP-1 (P-Ser591)
  • Gene Symbol: PTPN6 HCP PTP1C
  • Gene ID: 5777
  • Accession #: P29350
SHP2 (P-Tyr542)
  • Gene Symbol: PTPN11 PTP2C SHPTP2
  • Gene ID: 5781
  • Accession #: Q06124
Src (P-Tyr419)
  • Gene Symbol: SRC SRC1
  • Gene ID: 6714
  • Accession #: P12931
STAT1 (P-Ser727)
  • Gene Symbol: STAT1
  • Gene ID: 6772
  • Accession #: P42224
STAT2 (P-Tyr689)
  • Gene Symbol: STAT2
  • Gene ID: 6773
  • Accession #: P52630
STAT3 (P-Tyr705)
  • Gene Symbol: STAT3 APRF
  • Gene ID: 6774
  • Accession #: P40763
STAT5 (P-Tyr694)
  • Gene Symbol: STAT5A STAT5
  • Gene ID: 6776
  • Accession #: P42229
STAT6 (P-Tyr641)
  • Gene Symbol: STAT6
  • Gene ID: 6778
  • Accession #: P42226
TYK2 (P-Tyr1054)
  • Gene Symbol: TYK2
  • Gene ID: 7297
  • Accession #: P29597
   

Human JAK/STAT Pathway Phosphorylation Array Kit Components

  • Human JAK/STAT Pathway Phosporylation Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human JAK/STAT Pathway Phosphorylation Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
View AllClose

Additional Information

Product type:
Phosphorylation Array
Target:
EGFR (P-Ser1070)
Target:
JAK1 (P-Tyr1022)
Target:
JAK2 (P-Tyr1007/1008)
Target:
SHP-1 (P-Ser591)
Target:
SHP2 (P-Tyr542)
Target:
Src (P-Tyr419)
Target:
STAT1 (P-Ser727)
Target:
STAT2 (P-Tyr689)
Target:
STAT3 (P-Tyr705)
Target:
STAT5 (P-Tyr694)
Target:
STAT6 (P-Tyr641)
Target:
TYK2 (P-Tyr1054)
Reactivity:
Human
Application:
Multiplex Array
View AllClose